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Method for determining timosaponin BII content of traditional Chinese medicine composition

A technology of timosaponin and determination method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problems of different content determination methods and interference

Active Publication Date: 2012-10-24
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, timosaponin BⅡ is an important component in this medicinal material, and it is often used as a content detection index in a traditional Chinese medicine composition for quality control. The common determination methods are TLC scanning, high performance liquid chromatography, etc. , often different traditional Chinese medicine compositions, the content determination method of timosaponin BⅡ will be different, the reason is mainly due to the interference of different raw materials in the traditional Chinese medicine composition, therefore, the determination method of the same component in different traditional Chinese medicine compositions is very different It is difficult to use the same method for determination, and the determination method of each traditional Chinese medicine composition requires strict method screening to determine

Method used

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  • Method for determining timosaponin BII content of traditional Chinese medicine composition
  • Method for determining timosaponin BII content of traditional Chinese medicine composition
  • Method for determining timosaponin BII content of traditional Chinese medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Weight of raw materials: Astragalus 340kg, Rehmannia glutinosa 340kg, Caulis Spatholobus 340kg, Guizhi 113kg, Angelica 136kg, Chuanxiong 170kg, Lychee kernel 170kg, Anemarrhena 113kg, Sangzhi 113kg, Xu Changqing 102kg, Corydalis 91kg, Ephedra 68kg, Dilong 45kg;

[0078] Preparation:

[0079] 1) Take the traditional Chinese medicinal materials, select them separately, coarsely crush them into particles of about 5-10mm, and weigh them in proportion.

[0080] 2) Weigh Astragalus, Ephedra, Sangzhi, Corydalis Corydalis, Caulis Spatholobus, and Ligusticum Chuanxiong in proportion, add 10 times the amount and 8 times the amount of 60% ethanol respectively, and reflux extraction twice, and the extraction time is 2 and 1.5 hours respectively. Filter, combine the extracts, and discard the residue. Recover ethanol until there is no alcohol smell, concentrate under reduced pressure to a liquid extract with a relative density of 1.02-1.06 when measured at 60°C, and set aside.

[0...

Embodiment 2

[0093] Weight of raw materials: Astragalus 300kg, Rehmannia glutinosa 300kg, Caulis Spatholobus 300kg, Guizhi 80kg, Angelica 100kg, Chuanxiong 150kg, Litchi core 150kg, Anemarrhena 80kg, Sangzhi 80kg, Xu Changqing 80kg, Corydalis 70kg, Ephedra 50kg, Dilong 30kg ;

[0094] Preparation:

[0095] 1) Take the traditional Chinese medicinal materials, select them separately, coarsely crush them into particles of about 5-10mm, and weigh them in proportion.

[0096] 2) Weigh Radix Astragali, Ephedra, Sangzhi, Corydalis Corydalis, Caulis Spatholobus, and Rhizoma Chuanxiong in proportion, add 12 times, 10 times, and 8 times of 50% ethanol respectively, and reflux extraction 3 times, and the extraction time is 1 and 2 times respectively. ,3 hours. Filter, combine the extracts, and discard the residue. Recover ethanol until there is no alcohol smell, concentrate under reduced pressure to a liquid extract with a relative density of 1.02-1.06 when measured at 60°C, and set aside.

[009...

Embodiment 3

[0109] Weight of raw materials: Astragalus 400kg, Rehmannia glutinosa 400kg, Caulis Spatholobus 400kg, Guizhi 120kg, Angelica 150kg, Chuanxiong 200kg, Litchi core 200kg, Anemarrhena 120kg, Sangzhi 120kg, Xu Changqing 120kg, Corydalis 120kg, Ephedra 100kg, Dilong 60kg ;

[0110] Preparation:

[0111] 1) Take the traditional Chinese medicinal materials, select them separately, coarsely crush them into particles of about 5-10mm, and weigh them in proportion.

[0112] 2) Weigh Astragalus, Ephedra, Sangzhi, Corydalis Corydalis, Caulis Spatholobus, and Ligusticum Chuanxiong in proportion, add 10 times the amount and 12 times the amount of 70% ethanol respectively, and reflux extraction twice, and the extraction time is 1 hour and 3 hours respectively. Filter, combine the extracts, and discard the residue. Recover ethanol until there is no alcohol smell, concentrate under reduced pressure to a liquid extract with a relative density of 1.02-1.06 when measured at 60°C, and set aside....

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Abstract

The invention discloses a method for determining timosaponin BII content of a traditional Chinese medicine composition. The traditional Chinese medicine composition comprises astragalus mongholicus, dried rehmannia root, suberect spatholobus stem, cassia twig, Chinese angelica, ligusticum wallichii, lychee seeds, rhizoma anemarrhenae, mulberry twig, paniculate swallowwort root, Rhizoma Corydalis, ephedra and earthworm. The traditional Chinese medicine composition is used for clinical treatment on diabeteic peripheral neuropathy. Through the high performance liquid chromatography, the method determines the timosaponin BII content of the traditional Chinese medicine composition. The method is simple, fast, accurate and reliable and can be used for quality control of an intermediate and an end product of the traditional Chinese medicine composition.

Description

technical field [0001] The invention relates to a method for determining the content of timosaponin BII in a traditional Chinese medicine composition. Background technique [0002] CN1833703A discloses a medicine for treating diabetic peripheral neuropathy and a preparation method thereof. The Chinese medicine composition contains the raw material Anemarrhena anemarrhena, which is rich in steroidal saponins, biliprazones, flavonoids, lignans, Polysaccharides, organic acids and trace elements and other components. Among them, steroidal saponins are the main components, the amount of which is about 6% in the rhizomes, and there are many kinds. Among them, timosaponin BⅡ is an important component in this medicinal material, and it is often used as a content detection index in a traditional Chinese medicine composition for quality control. The common determination methods are TLC scanning, high performance liquid chromatography, etc. , often different traditional Chinese medic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/14
Inventor 王贵金裴彩云贾继明郑亚杰宋剑
Owner HEBEI YILING MEDICINE INST
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