Antifungal pharmaceutical composition
A technology of antifungal drugs and compositions, applied in the directions of antifungal agents, cyclic peptide components, etc., can solve the problems of liver cell damage, elevated liver enzymes, and high posaconazole, so as to reduce toxic reactions and reduce development. , the effect of reducing fungal resistance
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[0024] Embodiment 1, the interactive antifungal activity test of echinomycin and posaconazole
[0025] The interactive antifungal activity of echinomycin and posaconazole (Candida albicans SC5314) was tested by the checkerboard method. The specific method is:
[0026] Candida albicans SC5314 in RPMI1640 medium, 35°C, humidity 80%, 5% CO 2 Incubate under conditions. Dissolve echinomycin and posaconazole in DMSO to a concentration of 1 mg / mL, and store in a refrigerator until use. To determine the minimum inhibitory concentration (MIC), samples were tested using the two-fold dilution method. Specifically, refer to the National Committee for Clinical Laboratory Standards (NCCLS) M-27A protocol, that is, "Yeast Liquid Medium Dilution Antifungal Drug Susceptibility Test Protocol". Use liquid medium (RPMI1640) to prepare a series of diluted medicinal solutions by 2-fold dilution method, and then Candida albicans SC5314 cells (78 μl, ~1×10 4 ) were inoculated into a 96-well plat...
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