Crude dry extract of thunberg fritillary bulb total alkaloids

A technology of total alkaloids and fritillary fritillary is applied in the direction of drug combination, plant raw materials, plant/algae/fungus/moss components, etc. It can solve the problems of high cost, low extraction efficiency, and inapplicability to industrialized large-scale production, and achieve cost Low, high extraction efficiency, solvent-saving effect

Inactive Publication Date: 2012-11-14
宁波杰顺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The total alkaloid content in Fritillaria japonicus is very low, only containing 0.12%-0.25% (mass fraction), which brings special difficulties to the extraction of total alkaloids from Fritillaria japonicus
At present, there are not many literature studies on

Method used

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  • Crude dry extract of thunberg fritillary bulb total alkaloids
  • Crude dry extract of thunberg fritillary bulb total alkaloids
  • Crude dry extract of thunberg fritillary bulb total alkaloids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Take 150g of Fritillaria herb, add 4.5 times the amount of ethanol solution with a mass fraction of 70%, and extract in countercurrent at 30°C for 4 hours, filter and collect the filtrate, concentrate, add 1 / 4 times the mass fraction of the raw medicinal material to the concentrated solution to make it 2% hydrochloric acid, fully stirred, filtered, and the filtrate was collected, the residue was washed twice with a 2% hydrochloric acid solution, and the above filtrates were combined, and the filtrate was adjusted to pH 7 with a 5% NaOH solution, and the obtained solution was Ester extraction, collecting the ethyl acetate layer, concentrating the extract under reduced pressure to obtain the total alkaloid extract of Fritillaria japonicus, and drying the total alkaloids extract of Fritillaria japonicus at 70°C to obtain the total biological Alkali crude extract dry paste 790.3mg.

[0040] The "concentration under reduced pressure" is low-temperature vacuum concentration, ...

Embodiment 2

[0042] Take 150g of Fritillaria herb, add 4.5 times the amount of ethanol solution with a mass fraction of 75%, extract in countercurrent at 40°C for 6 hours, filter and collect the filtrate, concentrate, add 1 / 4 times the mass fraction of the raw medicinal material to the concentrated solution, and make the mass fraction 2% hydrochloric acid, fully stirred, filtered, and the filtrate was collected, the residue was washed twice with a hydrochloric acid solution with a mass fraction of 2%, and the above filtrates were combined, and the filtrate was adjusted to pH 8 with a NaOH solution with a mass fraction of 5%. Ester extraction, collecting the ethyl acetate layer, concentrating the extract under reduced pressure to obtain the total alkaloid extract of Fritillaria japonicus, and drying the total alkaloids extract of Fritillaria japonicus at 70°C to obtain the total biological Alkali crude extract dry paste 784.5mg.

[0043] The "concentration under reduced pressure" is low-tem...

Embodiment 3

[0045] Take 150g of Fritillaria herb, add 4.5 times the amount of ethanol solution with a mass fraction of 80%, and extract in countercurrent at 50°C for 5 hours, filter and collect the filtrate, concentrate, add 1 / 4 times the mass fraction of the raw medicinal material to the concentrated solution, and make the mass fraction 2% hydrochloric acid, fully stirred, filtered, and the filtrate was collected, the residue was washed twice with a 2% hydrochloric acid solution, and the above filtrates were combined, and the filtrate was adjusted to pH 9 with a 5% NaOH solution, and the obtained solution was Ester extraction, collecting the ethyl acetate layer, concentrating the extract under reduced pressure to obtain the total alkaloid extract of Fritillaria japonicus, and drying the total alkaloids extract of Fritillaria japonicus at 70°C to obtain the total biological Alkali crude extract dry paste 792.6mg.

[0046] The "concentration under reduced pressure" is low-temperature vacuum ...

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Abstract

The invention discloses a crude dry extract of thunberg fritillary bulb total alkaloids. The crude dry extract is extracted by using a countercurrent extraction method. The countercurrent extraction method includes the following steps of step one, taking and crushing thunberg fritillary bulbs, adding 65%-85% of an ethanol aqueous solution to the thunberg fritillary bulbs, subjecting the mixture to countercurrent extraction for 4-6 hours at the temperature of 30-60 DEG C, filtering the mixture, combining and collecting filter liquor and concentrating the filter liquor to obtain a concentrated solution; step two, adding muriatic acid to the concentrated solution, performing full stirring and filtering, collecting filter liquor, using a muriatic acid solution to wash residues for two times and combining the filter liquor; and step three, using potassium hydroxide with a mass fraction of 5% to alkalify the filter liquor obtained from the step two, regulating the potential of hydrogen (pH) value of the filter liquor to be 7.0-9.0, using ethyl acetate to extract the obtained solution and subjecting the extracted solution to decompression concentration to obtain the thunberg fritillary bulb total alkaloid extract. By means of the countercurrent extraction method, alkaloid ingredients can be fully extracted, and compared with existing technical methods, the countercurrent extraction method has the advantages of being high in extraction efficiency, low in cost, capable of saving solvents, applicable to industrial production and the like.

Description

technical field [0001] The invention relates to the technical field of extraction of effective components of Chinese medicinal materials, in particular to a dry cream of fritillary fritillaria total alkaloid crude extract. Background technique [0002] Fritillaria fritillaria belongs to the genus Fritillaria of Liliaceae. The dried bulbs of Fritillaria fritillaria are one of the traditional traditional Chinese medicines and are listed as one of the famous "Eight Flavors of Zhejiang". Phlegm, cough, swelling and other effects. The main active ingredients of Fritillaria are alkaloids, including Peyerine, Peyerine, Proto-Peilline, Fritillin, Isobeamine, etc. [0003] The total alkaloid content in Fritillaria japonicus is very low, only containing 0.12%-0.25% (mass fraction), which brings special difficulties to the extraction of total alkaloids from Fritillaria japonicus. At present, there are not many literature studies on the preparation method of Fritillaria fritillaria to...

Claims

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Application Information

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IPC IPC(8): A61K36/8966A61P11/00A61P29/00A61P11/10A61P11/14A61K125/00
Inventor 季进军
Owner 宁波杰顺生物科技有限公司
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