Method for detecting spirit by using chromatographic analysis of spirit
A gas chromatographic analysis, gas chromatographic technology, used in the analysis of materials, measuring devices, material separation, etc.
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Embodiment 1
[0076] Embodiment 1: chromatographic analysis of ethyl acetate in liquor
[0077] 1. Instrument configuration: packed injector, FID detector, controllable temperature program oven, computer and chromatographic workstation, gas chromatograph (GC-8600 type of Beifen Tianpu Instrument Technology Co., Ltd.).
[0078] 2. Experimental Conditions and Columns
[0079] Chromatographic column: 2m*Φ2mm packed column (stainless steel column), internal stationary phase: 20% DNP+7% Tween 80, and the carrier is Chromosorb W (AW) 80 mesh.
[0080] Injector temperature: 250 degrees. Injection volume 1uL.
[0081] Column temperature: 90 degrees, carrier gas: N 2 30mL / min.
[0082] Detector: 250 degrees, H 2 30mL / min, air 300mL / min.
[0083] 3. Sample to be tested: Hongchao Fen-flavor liquor
[0084] 4. Analysis method: first inject 1uL of liquor qualitative mixed standard sample No. 1 (see the table below) into the chromatograph, and obtain the qualitative template in the workstation. ...
Embodiment 2
[0104] Embodiment 2: ethyl hexanoate chromatographic analysis method in liquor
[0105] 1. Instrument configuration: capillary split injector, FID detector, controllable program temperature rise column oven, computer and chromatographic workstation, gas chromatograph (Shanghai Tianmei GC7900).
[0106] 2. Chromatographic column and experimental conditions:
[0107] Chromatographic column: Quartz capillary column PEG20M cross-linked type 30m*Φ0.32mm, film thickness: 0.5um;
[0108] Injector temperature: 230 degrees, injection volume: 0.8uL;
[0109] Column temperature program: 50 degrees (5min)-5 degrees / min-180 degrees (5min);
[0110] Detector temperature: 230 degrees, H 2 30mL / min, air: 300mL / min;
[0111] Carrier gas: high purity N 2 (purity: 99.99%), flow: 0.8mL / min, split: 80:1.
[0112] 3. Sample: Yingjia Liquor (Luzhou-flavor type)
[0113] 4. Analysis method: first inject 0.8uL of standard sample No. 2 (see the table below) of 31 qualitative components of liquo...
Embodiment 3
[0119] Embodiment 3: β-phenylethanol analysis method in liquor
[0120] 1. Instrument configuration: same as embodiment 2;
[0121] 2. Chromatographic column and experimental conditions: with embodiment 2;
[0122] 3. Sample: Pagoda Baijiu (rice flavor, 3 years);
[0123] 4. Analytical method: first inject 0.8uL of mixed standard sample No. 2 of 31 qualitative components of liquor into the chromatographic instrument, and obtain the qualitative template on the chromatographic workstation. Measure 0.1mL internal standard solution (1% n-butyl acetate) into a 10mL volumetric flask, fill to the mark with liquor sample, and shake well. Use a 1uL micro-syringe to draw 0.8uL plus internal standard sample and inject it into the chromatographic instrument, get the chromatogram of the sample on the workstation, and use the standard sample qualitative template to characterize the peaks of β-phenylethanol and other components according to the retention time. The contents of β-phenylethano...
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