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Detection kit for highly specific multiple genes related to movement functions and detection method thereof

A gene detection, high-specificity technology, applied in the field of high-specificity multiple motor function-related gene detection kits, can solve the problems of cumbersome operation, difficult interpretation of results, poor repeatability, etc., saving manpower, material resources and time, and high sensitivity. The effect of specific simultaneous detection

Active Publication Date: 2015-02-11
山东百福基因科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have different defects, such as cumbersome operation, difficult interpretation of results, poor repeatability, many false negatives and false positives, etc.

Method used

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  • Detection kit for highly specific multiple genes related to movement functions and detection method thereof
  • Detection kit for highly specific multiple genes related to movement functions and detection method thereof
  • Detection kit for highly specific multiple genes related to movement functions and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The kit of the present invention detects the DNA samples of 4 different individuals. The primers used for the detection of motor function-related genes were labeled with blue fluorescent dye, and the internal standard was labeled with red fluorescent dye.

[0056] 1. The 4 samples to be tested have all been sequenced and detected by using the technical method of "DNA extraction-PCR amplification-sequencing". Among them, the typing results of the four genes of ACE, ACTN3, CKMM, and EPO in sample No. 71-2 are: D / D, C / T, G / G, T / G, and the typing results of sample No. 74-2 are in order It is: I / I, C / T, A / A, T / G, the typing results of sample No. 73-4 are: D / D, T / T, A / A, T / T, and the typing results of sample No. 87 The typing results are as follows: D / D, C / C, A / G, T / G.

[0057] 2. Genomic DNA extraction from samples

[0058] Chelex extraction method: Cut off 1-3mm blood spot and put it in a 1.5mL centrifuge tube, add sdH 2 O 1mL, shake and centrifuge, discard the supernat...

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Abstract

The invention discloses a detection kit for highly specific multiple genes related to movement functions and a detection method thereof, belonging to the technical field of biological detection. Four mutation hotspots in four genes related to the movement functions can be detected simultaneously by the kit in 3h; and the kit contains a pre-amplification reagent, a post-amplification reagent and a primer mixture of the four mutation hotspots in the four highly specific amplified genes ACTN3, EPO, CKMM and ACE. With the four genes as detection objects and through the amplification of the hotspots, the capillary electrophoresis and the comparison to the allelic ladder, genetic typing can be performed and individuals with specific genotypes can be screened to provide a reference for sportsmen selection. The fluorescent labeling technology and the capillary electrophoresis are commonly applied to sportsmen selection for the first time so as to realize the high-sensitivity and high-specificity detection of the genes related to the movement functions and greatly save manpower, material resources and time.

Description

technical field [0001] The invention relates to a highly specific multiple motor function-related gene detection kit and a detection method for simultaneously detecting four motor function-related genes, belonging to the technical field of biological detection. Background technique [0002] With the completion of the Human Genome Project and the full development of the Post-Genome Project, the application of genetic engineering-led biotechnology in the field of sports has received widespread attention. The application of genetic engineering to athlete selection is a historical necessity and the trend of the times. It will fundamentally cause a revolution in the theory and methods of sports selection. Preliminary progress has been made in sports selection using the theory and methods of genetics. Multiple gene loci related to individual athletic ability have been discovered, and the application of genetic engineering in sports talent selection has broad and urgent prospects....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 步迅夏子芳
Owner 山东百福基因科技有限公司