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Microwave drying method of microbial starter culture

A microwave drying and microbial technology, applied in food science, fungi, bacteria, etc., can solve problems that have not been reported before, and achieve the effects of short drying time, low temperature and low energy consumption

Inactive Publication Date: 2013-09-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been no reports about the microwave drying method of microbial fermentation agents.

Method used

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  • Microwave drying method of microbial starter culture
  • Microwave drying method of microbial starter culture
  • Microwave drying method of microbial starter culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The microwave drying method example of embodiment 1 yeast starter

[0035] The Brettanomyces castellum isolated from the traditional production process of Mifa Cake was isolated and identified by the applicant (the strain number is ZSM-001, and it is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M207150, see China Publication Instructions Publication Number: CN101173223A, Publication Date: May 07, 2008), use PDA slant medium for activation and culture for three generations, then transfer to Erlenmeyer flask PDA liquid medium, and expand culture on a constant temperature shaker at 37°C for 48 hours , 4000r / min centrifugal concentration for 20min, collect the sludge. Add 20g of rice flour, 10g of sucrose, 10g of skimmed milk, and 10g of sodium alginate to 1000g of bacteria slime to make a bacteria-containing mixture with a moisture content of about 25%. After the ingredients are mixed and stirred evenly, use a granula...

Embodiment 2

[0038] The microwave drying method example of embodiment 2 saccharomycetes and lactic acid bacteria mixed starter

[0039] The Lactobacillus plantarum isolated and screened by the present application (the bacterial strain number is ZSM-002, is preserved in the China Type Culture Collection Center, the preservation number is CCTCC NO: M209127, see the publication specification of the Chinese invention patent application, the publication number is CN101691551A, and the publication date is April 07, 2010) and Brettanomyces castellum ZSM-001 (the same source as above), were activated and cultivated for three generations with MRS and PDA slant medium respectively, and then transferred to the Erlenmeyer flask MRS and PDA liquid medium respectively. After expanding the culture on a constant temperature shaking table at 37°C for 48 hours, centrifuge and concentrate at 4000r / min for 20min to collect the sludge. Take 900g of Brett yeast ZSM-001 sludge and 100g of Lactobacillus plantarum...

Embodiment 3

[0042] The microwave drying method example of embodiment 3 lactic acid bacteria and Staphylococcus ferment

[0043] Pediococcus pentosaceae (strain number: CICC22227, purchased from Beijing Beina Chuanglian Biotechnology Research Institute) and Staphylococcus xylosus (strain number: CICC10145, purchased from Beijing Beina Chuanglian Biotechnology Research Institute) used in this example Use MRS and NA slant medium to activate and culture for three generations, then transfer to Erlenmeyer flask liquid MRS and Erlenmeyer flask liquid NA medium respectively, expand culture on a constant temperature shaker at 37°C for 48 hours, centrifuge and concentrate at 4000r / min for 20min, collect Sludge. Take 500g of Pediococcus pentosaceae bacteria slime and 500g of Staphylococcus xylosus bacteria sludge. After mixing evenly, add 20g of peptone, 10g of white sugar, 10g of skimmed milk powder and 10g of lactose to 1000g of bacteria sludge to make a water content of about 28%. bacteria mixtu...

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Abstract

The invention belongs to the technical field of preparation of a microbial starter culture, and particularly relates to a microwave drying method of the microbial starter culture. The microwave drying method comprises the following steps of: (1) inoculating a stored strain on a slant culture medium to activate, inoculating activated strain on the slant culture medium and continuously repeating for three times to obtain activated strain; (2) inoculating the activated strain in a liquid culture medium, and culturing for 24-48 hours to obtain enlarge culture solution of the strain; (3) filtering or centrifugally concentrating the enlarge culture solution of the previous step and collecting microorganism mud; (4) adding a protecting agent and a filling agent into the microorganism mud to obtain microorganism-containing mixture with water content of 13-35 percent; (5) granulating the microorganism-containing mixture by using the granulating machine to obtain a granular microorganism-containing substance; and (6) treating the granular microorganism-containing substance by using microwave with dosage of 0.3-3 kW / kg for 2-60 minutes to obtain the microbial solid-state starter culture. Compared with the prior art, the drying time is short, the energy consumption is low, the temperature in a drying process is low, and the activity of the microorganism is favorably kept and the quality of the starter culture is favorably improved.

Description

technical field [0001] The invention belongs to the technical field of food processing and is related to the technical field of drying food microbial starters. The invention specifically relates to a microwave drying method of microbial starters. Background technique [0002] Microorganisms play an important role in the human diet. The excellent flavor and functional ingredients of most foods are formed under the fermentation of microorganisms, such as steamed bread, bread, yogurt, cured fish, bacon, pickles and other foods. The traditional production method of this kind of fermented products is through natural fermentation, but the production cycle of natural fermentation is long, the efficiency is low, and the production process is difficult to control. Now the production of some fermented products has been gradually replaced by inoculation of microbial starter (Tan Rucheng et al. Inoculation of plants Effects of fermentation of Lactobacillus and Pediococcus pentosaceus on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/20A23L1/03A23L29/00
Inventor 赵思明谢静熊善柏熊青鲍芳芳方炎鹏
Owner HUAZHONG AGRI UNIV