Kit for detecting mycobacterium tuberculosis based on loop-mediated isothermal amplification, and primers

Abstract

The invention discloses a kit for detecting mycobacterium tuberculosis based on a loop-mediated isothermal amplification technology, and primers applied to the kit. The kit is low in detection cost, convenient to use, and high in sensitivity, and can detect mycobacterium tuberculosis quickly and efficiently. The sequences of the primers are shown as sequence numbers SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4; and besides the primers, the kit also comprises 12.5mu l of amplification system premixture, 1 mu l of primer F3 at the concentration of 5 to 15pmol / mu l, 1mul of primer B3 at the concentration of 5 to 15 pmol / mu l, 1 mu l of primer FIP at the concentration of 20 to 40 pmol / mu l, 1 mu l of primer BIP at the concentration of 20 to 40 pmol / mu l, and 2.5 mu l of sterile double distilled water. By using the kit, the mycobacterium tuberculosis is detected by loop-mediated isothermal amplification, so that detection cost can be reduced greatly; and the kit can detect mycobacterium tuberculosis quickly and efficiently, and is high in sensitivity. The kit can be applied to the field of detection of mycobacterium tuberculosis.

Description

technical field [0001] The invention relates to the detection technology of mycobacterium tuberculosis, in particular to a detection kit for detecting mycobacterium tuberculosis by loop-mediated isothermal amplification and primers thereof. Background technique [0002] Mycobacterium Tuberculosis (TB) is abbreviated as tubercle bacilli. As early as 1882, German bacteriologist Guo Huo (Robert Koch, 1843-1910) had proved that Mycobacterium tuberculosis is the pathogenic bacteria of tuberculosis. Mycobacterium tuberculosis can invade various tissues and organs throughout the body, but the most common infection is the lungs. With the continuous development of anti-tuberculosis drugs and the improvement of sanitary living conditions, the morbidity and mortality of tuberculosis once dropped sharply. After the 1980s, due to factors such as the emergence of AIDS and drug-resistant strains of Mycobacterium tuberculosis, the application of immunosuppressants, drug abuse, poverty, an...

AI Technical Summary

Problems solved by technology

Microscopic examination of sputum smear is easy to operate, quick to produce results, and low in cost, but it is only based on the acid resistance characteristics, so it cannot be used as the basis for the specific identification of Mycobacterium tuberculosis

(2) Sputum tuberculosis culture, the results are highly reliable, and tuberculosis drug susceptibility test can be done, but it takes 6-8 weeks, and the application is limited

Due to the complexity of current gene detection technology and the high infectivity of Mycobacterium tuberculosis, the genetic detection of Mycobacterium tuberculosis not only requires expensive instruments and equipment, but also has high requirements for laboratory conditions. Rapid diagnosis has caused many difficulties, which is not conducive to primary medical epidemic prevention institutions and on-site rapid detection

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