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A kind of preparation method of citric acid fermented liquid

A technology for citric acid fermentation broth and fermentation broth, which is applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of long mycelium and easily sticky fermentation broth, and achieves the maintenance of pH value and fermentation power. The effect of reducing consumption and improving quality

Active Publication Date: 2015-09-02
RIZHAO JINHE BOYUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above problems, the present invention proposes a preparation method of citric acid fermented liquid, which solves the problems such as easy stickiness of fermented liquid and long hyphae in the production of citric acid, improves the transfer efficiency of oxygen in the fermentation process, and reduces the stirring time. Power consumption increases the benefit of sugar slag protein feed and improves the quality of fermentation broth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing citric acid fermentation broth is characterized in that the steps are:

[0028] (1) Corn flour is prepared by crushing corn, and the 60 mesh transmittance rate of crushed corn flour is 65%;

[0029] (2) The corn flour is mixed with water to adjust the mass fraction to 10% and the pH is 5.6, and high temperature resistant α-amylase is added to it, and 15 enzyme units of high temperature resistant α-amylase are added to each gram of corn flour;

[0030] (3) The corn flour slurry after mixing is transported to the jet liquefaction device for two consecutive jet liquefaction processes for liquefaction. The first jet liquefaction temperature is 96°C, and the second jet liquefaction temperature is 130°C. Maintained in the tank for 1 hour, the liquefied DE value is 10%;

[0031] (4) Take the liquefied liquid that accounts for 5% of the total volume of the final fermentation broth in step (3), put it into a seed tank, sterilize at 100°C for 20 minutes, cool to 35...

Embodiment 2

[0038] A method for preparing citric acid fermentation broth is characterized in that the steps are:

[0039] (1) Corn flour is prepared by crushing corn, and the 60 mesh transmittance rate of crushed corn flour is 75%;

[0040] (2) Add water to the corn flour to adjust the pulp to a mass fraction of 35% and a pH of 7.8, and add heat-resistant alpha-amylase to it, adding 25 enzyme units of high-temperature alpha-amylase per gram of corn flour;

[0041] (3) The corn flour slurry is transported to the jet liquefaction device to be liquefied through two consecutive jet liquefaction processes. The first jet liquefaction temperature is 95°C, and the second jet liquefaction temperature is 125°C. Maintained in the tank for 2 hours, the liquefied DE value is 35%;

[0042] (4) Take the liquefied liquid that accounts for 10% of the total volume of the final fermentation broth in step (3), put it into a seed tank, sterilize at 80°C for 15 minutes, cool to 38°C, and insert the Aspergillus niger s...

Embodiment 3

[0049] A method for preparing citric acid fermentation broth is characterized in that the steps are:

[0050] (1) Corn flour is prepared by crushing corn, and the 60 mesh transmittance rate of crushed corn flour is 70%;

[0051] (2) Add water to the corn flour to adjust the pulp to a mass fraction of 15% and a pH of 6.7, and add heat-resistant alpha-amylase to it, adding 5 enzyme units of high-temperature alpha-amylase per gram of corn flour;

[0052] (3) The mashed corn flour slurry is transported to the jet liquefaction device for two consecutive jet liquefaction processes for liquefaction. The first jet liquefaction temperature is 98°C, and the second jet liquefaction temperature is 126°C. Keep in the tank for 4 hours, the liquefied DE value is 25%;

[0053] (4) Take the liquefied liquid that accounts for 6% of the total volume of the final fermentation broth in step (3), put it into a seed tank, sterilize at 121°C for 30 minutes, cool to 36°C, and plug in the Aspergillus niger spo...

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PUM

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Abstract

The invention relates to a preparation method for citric acid fermentation broth. The preparation method is characterized in that corn is used as a raw material, continuous injection liquefaction is carried out twice, and diammonium phosphate, ammonia water and ammonium citrate are added in the process of fermentation for the first time to prepare a composite nitrogen source nutrient solution for fermentation. The problems of easy tackifying of fermentation broth, long mycelia and the like in conventional production of citric acid are overcome; transmission efficiency of oxygen in the fermentation process is improved; power consumption in stirring is reduced; benefits of a sugar residue protein feed are increased; the quality of the fermentation broth is improved; and comprehensive competitive power of an enterprise is strengthened.

Description

Technical field [0001] The invention relates to a method for preparing citric acid fermentation broth, in particular to a method for preparing citric acid fermentation broth by supplementing an inorganic nitrogen source. Background technique [0002] Citric acid is an organic acid widely used in food, medicine and chemical industries. With the development of the economy, the demand for citric acid in all walks of life has steadily increased, and the challenges and opportunities faced by citric acid manufacturers are increasing. [0003] At present, the main problems in the production of citric acid are the high concentration of the fermentation broth and the high viscosity of the fermentation broth, which is not conducive to the mass transfer of dissolved oxygen, resulting in high energy consumption of the fermentation stirring motor and air compressor, which seriously restricts the rapid development of the citric acid industry. development of. Summary of the invention [0004] In...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/48C12R1/685
Inventor 寇光智刘剑雄周一江安丰民裴振洪焦光明
Owner RIZHAO JINHE BOYUAN BIOCHEM