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Method for rapidly determining PienTzeHuang quality by using near-infrared spectroscopy

A near-infrared spectroscopy and rapid determination technology, applied in the field of medicine, can solve problems such as polluting the environment, unable to quickly monitor the quality of Pien Tze Huang, and complicated processing

Active Publication Date: 2014-03-05
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quality detection methods of Pien Tze Huang national standards mostly use thin-layer chromatography, gas chromatography, high-performance liquid chromatography, etc., but these methods have problems such as complicated sample pretreatment, long detection time, high detection cost, and environmental pollution. Offline detection, unable to quickly monitor the quality of Pien Tze Huang

Method used

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  • Method for rapidly determining PienTzeHuang quality by using near-infrared spectroscopy
  • Method for rapidly determining PienTzeHuang quality by using near-infrared spectroscopy
  • Method for rapidly determining PienTzeHuang quality by using near-infrared spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Methodological research on the rapid determination of the quality of Pien Tze Huang using near-infrared spectroscopy

[0039] 1. Instruments and reagents:

[0040] Fourier transform near-infrared spectrometer Bruker MPA; instrument accessories: OPUS processing software; Agilent high performance liquid chromatograph 1200.

[0041] Notoginsenoside R 1 , Ginsenoside Rg 1 and ginsenoside Rb 1 (National Institute for the Control of Pharmaceutical and Biological Products, batch numbers are 110745-200617; 110703-200726; 110704-200921), Pien Tze Huang 78 batches, acetonitrile is chromatographically pure, and other reagents are analytically pure.

[0042] 2. Method

[0043] (1) Determination of notoginseng saponin R in 78 batches of Pien Tze Huang tablets by HPLC 1 , Ginsenoside Rg 1 and ginsenoside Rb 1 Content

[0044] 1. Chromatographic conditions

[0045] The chromatographic column is a Welch Materials C18 chromatographic column (250 mm×4.6 mm 5 μm); the de...

Embodiment 2

[0087] Example 2 The quality of Pien Tze Huang lozenges quickly measured by near-infrared spectroscopy

[0088] (1) Collect 80 batches of representative Pien Tze Huang tablets as the training set.

[0089] (2) HPLC (instrument: Agilent HPLC 1200) Determination of notoginsenoside R in Pien Tze Huang Tablets in the training set 1 , Ginsenoside Rg 1 and ginsenoside Rb 1 content.

[0090]Chromatographic conditions: the chromatographic column is a Welch Materials C18 chromatographic column (250mm×4.6mm 5μm); the detection wavelength is 203nm; the flow rate is 1ml / min; the column temperature is 25°C. Mobile phase: acetonitrile-water (phase A is water, phase C is acetonitrile. 0~32min, A is 21%, C is 79%; 32~65min, A is 21%→40%, C is 79%→60% ). The theoretical plate number should not be less than 2500 based on the calculation of the notoginseng saponin R1 peak.

[0091] Preparation of reference solution: accurately weigh ginsenoside Rg 1 Reference substance, ginsenoside Rb 1 ...

Embodiment 3

[0099] Example 3 The quality of Pien Tze Huang powder is quickly measured by near-infrared spectroscopy

[0100] (1) Collect 100 batches of representative Pien Tze Huang tablets as the training set.

[0101] (2) HPLC (instrument: Agilent HPLC 1200) Determination of notoginsenoside R in Pien Tze Huang Tablets in the training set 1 , Ginsenoside Rg 1 and ginsenoside Rb 1 content.

[0102] Chromatographic conditions: the chromatographic column is a Welch Materials C18 chromatographic column (250mm×4.6mm 5μm); the detection wavelength is 203nm; the flow rate is 1ml / min; the column temperature is 25°C. Mobile phase: acetonitrile-water (phase A is water, phase C is acetonitrile. 0~32min, A is 21%, C is 79%; 32~65min, A is 21%→40%, C is 79%→60% ). The theoretical plate number should not be less than 2500 based on the calculation of the notoginseng saponin R1 peak.

[0103] Preparation of reference solution: accurately weigh ginsenoside Rg 1 Reference substance, ginsenoside Rb ...

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Abstract

The invention discloses a method for rapidly determining PienTzeHuang quality by using near-infrared spectroscopy. The method comprises the steps that: (1) representative PienTzeHuang samples are collected as a training set; (2) quality indicators of the PienTzeHuang samples in the training set are determined through a high-performance liquid chromatography method; (3) near-infrared spectral data of the PienTzeHuang samples in the training set is determined; the near-infrared spectral data is pretreated; and spectral data obtained after pretreatment is adopted as variables; (4) the spectral data obtained after pretreatment is associated with the quality indicators, such that a correction model is established; and the correction model is verified; and (5) qualities of PienTzeHuang to be tested are determined by using the verified correction model. With the method, whether a sample to be tested is suspicious can be rapidly determined. The method is suitable for rapid screening of counterfeit and inferior fake goods. Also, the method can be used for evaluating quality differences and stability of each batch of PienTzeHuang. The method has the advantages of short detection time, high speed, accurate determination, and capability of online detection. With the method, manpower and material resources can be greatly saved, and substantial economic and social benefits are provided.

Description

technical field [0001] The invention relates to a method for quickly measuring the quality of Pien Tze Huang, in particular to a method for quickly measuring the quality of Pien Tze Huang by using near-infrared spectroscopy, which belongs to the field of medicine. Background technique [0002] Pien Tze Huang is a lozenge made from bezoar, Panax notoginseng, snake gall, musk, etc. It has a slightly fragrant, bitter and slightly sweet taste. It has the effects of anti-inflammatory, cooling and detoxifying, reducing swelling and relieving pain, etc. It has curative effect on acute hepatitis, ophthalmia, otitis, carbuncle and furuncle, unknown swollen poison, bruises and all pain and fever caused by inflammation; and can inhibit Growth of cancer cells in the digestive system. Pien Tze Huang is a national first-class protected traditional Chinese medicine. Its unique curative effect has been recognized by domestic and foreign markets. At the same time, there are many counterfeit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/359G01N21/3563G01N21/3577
Inventor 潘杰陈纪鹏于娟陈啟兰
Owner ZHANGZHOU PIEN TZE HUANG PHARM