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High-efficiency method for inducing pinellia in vitro bulb

An isolated and corm technology, which is applied in the field of efficient induction of Pinellia in vitro, can solve the problems of cumbersome tissue culture procedures, unsuitability for large-scale production, and low survival rate of transplanting, and achieve the application value of large-scale production and planting Convenient, easy-to-prepare effects

Inactive Publication Date: 2014-07-16
遵义市龙驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned technical problems, the present invention adopts a high-efficiency induction method of pinellia petiole embryoid body and isolated corm, which solves the problem that the transplanting survival rate of pinellia tissue rapid propagation system is not high in the prior art; The tissue culture procedure of Pinellia is cumbersome and not suitable for large-scale production; ensuring the induction rate of Pinellia embryoid bodies can also promote the formation of isolated corms; finally solve the problem of the contradiction between Pinellia resource protection and market demand

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  • High-efficiency method for inducing pinellia in vitro bulb

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Embodiment 1

[0025] Cut Pinellia petiole into 1 cm as explants, inoculate in embryoid body induction medium (MS+6-BA0.3mg / L+IBA0.2mg / L+commercially available white sugar 3%+agar powder 0.45%; pH5.8 ), under the conditions of 27°C, 1200Lx light, and 12h / d for 30 days, all explants induced embryoid bodies, and the number of embryoid bodies in each explant was more than 20; The corm was inoculated in the isolated bulb induction medium (1 / 2MS+6-BA 0.2mg / L+NAA 0.1mg / L+commercially available white sugar 3%, pH 5.8) in a glass bottle at 27°C under natural light conditions indoors. After 45 days of submerged culture, the embryoid body directly forms an isolated corm. After washing the culture medium attached to the isolated corm, dry the surface water in the shade, and directly sow it in the field. At 28°C, it will start to germinate in about 7 days. The germination rate is as high as 95%.

Embodiment 2

[0027] Cut Pinellia petiole into 0.8 cm as explants, inoculate in embryoid body induction medium (MS+6-BA0.2mg / L+IBA0.15mg / L+commercially available white sugar 6%+agar powder 0.45%; pH5. 9), after 33 days of culture at 26°C, 1200Lx light, and 12h / d, all explants induced embryoid bodies, and the number of embryoid bodies in each explant was more than 30; the induced Embryoids were inoculated in isolated corm induction medium (1 / 2MS+6-BA 0.15mg / L+NAA 0.1mg / L+commercially available white sugar 3%, pH 5.9) in a plastic bag at 26°C under natural light indoors After 43 days of culture under the same conditions, the embryoid bodies directly formed isolated corms. After washing the culture medium attached to the isolated corms, dry the surface water in the shade, and directly sow them in the field. At a temperature of 30°C, it will start in about 7 days. Germinate, the germination rate is as high as 96%.

Embodiment 3

[0029] Cut Pinellia petiole into 1.2 cm as explants, inoculate in embryoid body induction medium (MS+6-BA0.1mg / L+IBA0.5mg / L+commercially available white sugar 9%+agar powder 0.45%; pH6. 0), 25°C, 1200Lx light, 12h / d for 35 days, all explants induced embryoid bodies, and the number of embryoid bodies in each explant was more than 20; The embryoid bodies were inoculated in the isolated corm induction medium (1 / 2MS+6-BA 0.1mg / L+NAA 0.1mg / L+commercially available white sugar 3%, pH 6.0) in a plastic bag, at 25°C, under natural light indoors After 40 days of culture under the same conditions, the embryoid body directly forms an isolated corm. After washing the culture medium attached to the isolated corm, dry the surface water in the shade, and directly sow it in the field. At 28°C, it will start in about 7 days. Germinate, the germination rate is as high as 95%.

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Abstract

The invention provides a high-efficiency method for inducing pinellia in a vitro bulb. The method mainly comprises the following steps of: (1) selecting petiole as an explant; (2) inductively culturing embryoid; (3) inductively culturing the in vitro bulb; and (4) transplanting or storing the in vitro bulb in the field. According to the method, the explant can be induced to directly differentiate the embryoid, and the embryoid can be induced to directly form the in vitro bulb. The high-efficiency method for inducing the pinellia in the vitro bulb is simple and rational in process, convenient for preparation of a culture medium, high in inductivity, strong in fecundity and high in survival rate for in vitro bulb transplanting, can be directly cultivated or stored without special acclimatization to realize connection between pinellia seedling production and manual large-scale cultivation, and has higher production and application values.

Description

technical field [0001] The invention relates to a traditional Chinese medicine tissue culture method, in particular to a high-efficiency induction method for isolated pinellia corms. Background technique [0002] The corm of pinellia is used as medicine, which has various effects such as drying dampness and resolving phlegm, reducing adverse reactions and relieving cough, reducing swelling and dissipating stagnation, anti-early pregnancy, anti-tumor, etc. It is a unique traditional Chinese medicinal material in my country. With the deepening of pharmacological research, the market demand of pinellia is increasing. Pinellia pinellia has always been dominated by wild resources. Due to excessive excavation and intensive cultivation of the land, the resources of pinellia pinellia are almost exhausted. Although the artificial cultivation of Pinellia has been promoted, due to the long-term use of small bulbs as bulbs, the virus infection continues to accumulate in the bulbs, resu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘红美方小波梁大勇
Owner 遵义市龙驰生物科技有限公司