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Primer for detecting activity of wheat grain lipoxidase and application thereof

A technology of oxidase activity and lipoxygenase, applied in the fields of application, botanical equipment and methods, and microbial determination/inspection, etc., can solve the problem of low LOX activity and achieve reliable results.

Inactive Publication Date: 2014-04-02
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, existing studies have shown that LOX activity is low, which is beneficial to enhance the storage resistance of crops such as wheat

Method used

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  • Primer for detecting activity of wheat grain lipoxidase and application thereof
  • Primer for detecting activity of wheat grain lipoxidase and application thereof
  • Primer for detecting activity of wheat grain lipoxidase and application thereof

Examples

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Effect test

Embodiment 1

[0041] Example 1. Acquisition and application of molecular markers related to wheat grain lipoxygenase activity

[0042] 1. Determination of wheat grain lipoxygenase activity traits

[0043] The 148 wheats listed in Table 1 were used as experimental materials, and the LOX activity in wheat grains was measured with reference to the spectrophotometer method of Larisa Catod and modified appropriately. The specific operation is as follows:

[0044] (1) Substrate preparation: 0.5ml Tween 20 was dissolved in 10ml boric acid buffer solution with pH 9.0 concentration of 0.05mol / L (recipe: take 0.76g Na 2 BO 4 10H 2 O, 0.124g H 3 BO 3 , dilute to 200ml with double distilled water) and mix evenly, then add 0.5ml linoleic acid drop by drop, mix well to form an emulsion, add 1.3ml NaOH aqueous solution with a concentration of 1mol / L until the solution is clear, then add 90ml concentration It is 0.05mol / L boric acid buffer solution with pH 9.0, adjusted to 200ml after adjusting the p...

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Abstract

The invention discloses a primer for detecting the activity of wheat grain lipoxidase and application of the primer. The primer is a primer pair composed of a single-stranded deoxyribonucleic acid (DNA) as shown in Sequence 1 in a sequence table and a single-stranded DNA as shown in Sequence 2 in the sequence table. Experiments show that among wheat varieties of three amplified bands, namely 475bp, 677bp and 786bp, 98.29% of the wheat varieties are wheat varieties of high-fat oxidase activity, and among wheat varieties of two amplified bands, namely the 475bp and the 786bp, 80.06% of the wheat varieties are wheat varieties of low-fat oxidase activity. The primer for detecting the activity of wheat grain lipoxidase is reliable, simple, convenient and practical and has important application prospects in wheat germplasm evaluation and breeding marker-assisted selection, and at the same time, a reference is provided for cultivation of storage-resistant wheat varieties.

Description

technical field [0001] The invention relates to a pair of primers for detecting wheat grain lipoxygenase activity and application thereof. Background technique [0002] Wheat is one of the main grain storage crops in my country, and good grain quality is of great significance to food security. Under normal storage conditions, wheat will gradually age and deteriorate from the second year, especially in high-temperature and high-humidity areas in the south. There are many factors affecting the storage quality of wheat, including climatic conditions during the harvest season, external environmental conditions such as drying, transportation, and storage, and internal factors such as lipoxygenase activity, and the level of lipoxygenase activity is the main factor that directly affects the storage resistance of wheat. internal reason. Therefore, the development of molecular markers of LOX genes and the use of molecular breeding methods to breed storage-resistant wheat varieties ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11A01G1/00
Inventor 姚大年吴萍司红起张文明郑文寅
Owner ANHUI AGRICULTURAL UNIVERSITY