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Method for efficiently acquiring grape alga single cells and application thereof

A single-cell, grape algae technology, applied in the field of microorganisms, can solve the problems of low production efficiency, large cell damage, and low cell quality, and achieve the effects of low cost, high cell activity, and low light energy utilization

Active Publication Date: 2015-04-22
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional methods for obtaining single cells from cell populations mainly include mechanical disruption, ultrasonic disruption, and enzymatic hydrolysis. However, these methods often cause greater damage to cells, resulting in low quality cells, high production costs, and low production efficiency. It is often not possible to obtain single cells efficiently, and the obtained single cells are often a mixture of single cells and protoplasts, of which broken tissues occupy the majority

Method used

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  • Method for efficiently acquiring grape alga single cells and application thereof
  • Method for efficiently acquiring grape alga single cells and application thereof
  • Method for efficiently acquiring grape alga single cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Separation of botrytis single cell from extracellular tissue

[0042] The technical scheme of this embodiment is as figure 1 As shown, the specific steps are:

[0043] The cultivated algae bodies are collected, and the surface moisture of the algae bodies is removed at 5°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C or 40°C respectively. When the amount of algae to be treated is small, clean absorbent paper, desiccant or other water-absorbing materials can be used to remove part of the water on the surface of the algae cells; when dealing with a large number of algae, the collected algae can be placed in a ventilated and dry place Or use low-temperature drying equipment to remove part of the water on the cell surface.

[0044] Put the treated botrytis algae into sterilized medium or distilled water, the single cell of botrytis is naturally separated from the extracellular tissue, and becomes a mixture of single cell and extracellular tissue (see figure 2 ). Among...

Embodiment 2

[0046] Embodiment 2 obtains sterile botrytis single cell

[0047] The technical scheme of this embodiment is as image 3 As shown, the specific steps are as follows:

[0048] 1. Collect 100ml of cultured botrytis algae with a 300-mesh sieve, and use sterilized qualitative filter paper at 25°C to remove the water on the surface of the algae cells;

[0049] 2. Take the botrytis algae whose surface has been dried, put it into a sterilized Erlenmeyer flask containing 50ml of BG11 medium, and let it stand for 15 minutes, during which time shake the Erlenmeyer flask more than 3 times. After 15 minutes, most of the algae cells have been separated from the colony. Under aseptic conditions, use a sterile 300-mesh sieve to remove extracellular tissue, collect the filtrate, and obtain a highly active sterile botrytis unicellular algae liquid.

[0050] The composition of BG11 medium is shown in Table 1.

[0051] Table 1BG11 medium formula

[0052]

[0053] *A 5 Composition of trace ...

Embodiment 3

[0057] Example 3 Obtaining Extracellular Tissue and Extracting Oil

[0058] The technical scheme of this embodiment is as Figure 4 As shown, the specific steps are as follows:

[0059] 1. Collect the cultivated botrytis algae, and use clean and sterilized absorbent paper to remove the water on the surface of the algae cells at 20°C;

[0060] 2. Take the botrytis algae whose surface has been dried, put it into the sterilized medium for resuspension; at this time, the single cell of botrytis is separated from the extracellular tissue, and it is a mixture of single cell and extracellular tissue.

[0061] 3. Collect the botrytis extracellular tissue by filtering with a 300-mesh sieve, and freeze-dry the extracellular tissue;

[0062] 4. Take a certain amount of freeze-dried botrytis extracellular tissue and put it in a small glass bottle with a volume of 15-20ml with a Telfnon screw cap, add 5-10ml of n-hexane to ultrasonic for 10-30min, centrifuge at 3500 rpm, and collect n-h...

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Abstract

The invention discloses a method for efficiently acquiring grape alga single cells and an application thereof. The method comprises the step of removing partial moisture on the surface of wet alga to let water content of the wet alga be 60-80%. The method has the advantages of simpleness, easiness in operation, low cost, and efficient acquisition of high-activity grape alga single cells, and simultaneously oil extraction is easily carried out on the acquired extracellular tissues.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for efficiently obtaining single cells of botrytis and its application. Background technique [0002] With the shortage of global fossil energy and the environmental problems caused by the use of fossil energy, it is imperative to find new alternative energy. Microalgae have the characteristics of large biomass, short growth cycle, and high oil content. 2 Both emission reduction and biofuel development have broad development prospects. [0003] There are two main types of biofuels that can be developed using microalgae. One is the use of fatty acids synthesized by microalgae to produce biodiesel through esterification. Because most microalgae can accumulate a large amount of oil through fatty acid synthesis, this type of research and development is currently mostly used The other is to use alkanes synthesized by microalgae to produce bio-aviation kerosene. In microalgae, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C11B1/00C10L1/02C12R1/89
Inventor 陈传红张凯吴洪
Owner ENN SCI & TECH DEV
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