Antibacterial agents
An antibacterial composition, the technology of Methylobacterium, applied in the direction of bactericide, biocide, biocide, etc.
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[0075] Hereinafter, the present invention will be described in further detail based on examples.
reference example 1
[0076] Reference Example 1 Analysis of pathogenic bacteria of pink dirt
[0077] (1) Analysis of pathogenic bacteria of pink dirt by SEM observation
[0078] From the bathrooms of 14 families, the pink dirt in 39 sample bathrooms was wiped with a toothpick, suspended and fixed with 2.5% glutaraldehyde solution, washed with 100mM phosphate buffer solution of pH 7.6, and carried out with an ascending gradient ethanol system dehydration. After further freeze-drying and vapor-depositing platinum and vanadium on the surface, observation was performed at 7 to 15 kV using a scanning electron microscope (SEM) (S4300SE / N, Hitachi). An example of SEM observations is shown in figure 1 .
[0079]In all the samples, almost all the bacteria had a size of 3 μm or less, and no bacteria of 5 μm or more were observed. That is, in all the samples, the bacteria having the size and shape of the bacteria predominated, while the bacteria having the yeast size did not. Therefore, Rhodotorula ( ...
reference example 2
[0090] Reference example 2 Methylobacterium ( Methylobacterium ) bacteria resistant to antimicrobial agents
[0091] The mesophilic Methylobacterium mesophilus ( M. mesophilicum ) The cells of FP4 were cultivated overnight at 30°C using PDA plate medium (Difco), and the mesophilic Methylobacterium mesophilus ( M. mesophilicum ) FP4 cells were suspended in other potato dextrose liquid medium (PotatoDextrose Broth, PDB) and cultured with shaking at 30°C for 3 days. This was centrifuged (7000 g x 5 min.) to remove the supernatant, and then suspended with an equal amount of physiological saline. Afterwards, centrifuge again (7000g×5min.), and prepare the bacterial solution with OD0.8 with physiological saline. 1 ml of an octylbenzyldimethylammonium chloride solution (SANISOL08, manufactured by Kao Corporation) diluted with sterilized water was added to 10 μl of the bacterial solution, and allowed to contact for 5 minutes. Then dilute octylbenzyldimethylammonium chloride was...
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