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Process for extracting enteromorpha protein by enzymic method

A technology of enzymatic extraction and Enteromorpha, which is applied in the direction of protein food components, protein components of waste, protein composition of microorganisms, etc., can solve the problem of changing the nutritional characteristics of Enteromorpha protein, the destructiveness of Enteromorpha protein, and the loss of nutrients and other problems, to achieve the effect of reducing production cost, high purity and simple process

Inactive Publication Date: 2013-04-03
QINGDAO AIHUALONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the extraction and separation of Enteromorpha protein, and the laboratory mainly uses the acid-base method for extraction. Cystine and serine combine to form lys-alanine, which is not only toxic but also causes loss of nutrients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Dehydrate and dry the fresh Enteromorpha until the water content is less than 5%, pulverize it, and refine it into powder; take 1Kg of Enteromorpha powder and add 50 times the mass of water, stir evenly, and adjust the pH to 7.0 with 6mol / L sodium hydroxide solution; Add 7.5g of papain to the mixture, hydrolyze at 50°C for 2 hours, then add 2.5g of complex protease, continue to hydrolyze for 8 hours, cool at room temperature after the reaction, and centrifuge at 5000 rpm for 20 minutes. Take the supernatant and carry out spray drying, the air inlet temperature is 90°C, and the air outlet temperature is 45°C. After drying, 120.7g of light green enteromorpha protein is obtained. The protein content in the enteromorpha protein is 75.7%, and the protein yield is 54.2% %.

Embodiment 2

[0018] Dehydrate and dry fresh Enteromorpha until the water content is less than 5%, pulverize it, and refine it into powder; take 1Kg Enteromorpha powder and add 50 times the mass of water, stir evenly, and adjust the pH to 8.0 with 6mol / L potassium hydroxide solution; Add 15g of papain to the mixture, hydrolyze at 60°C for 4 hours, then add 5g of complex protease, continue to hydrolyze for 4 hours, cool at room temperature after the reaction, and centrifuge at 5000 rpm for 20 minutes. Get supernatant and carry out spray-drying, drying condition is the same as embodiment 1, after drying, obtain 123.4g light green enteromorpha protein, and protein content is 82.6% in this enteromorpha protein, and protein yield is 67.2%.

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PUM

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Abstract

The invention discloses a process for extracting enteromorpha protein by an enzymic method. The process comprises the following steps of 1. taking enteromorpha powers as raw materials; 2. adding water into the raw materials, mixing uniformly and regulating the pH value; 3. adding papain and compound protease into the mixture for extracting; 4. cooling at room temperature after reaction, and then carrying out centrifugal separation on the mixture; and 5. taking supernate, concentrating and drying, thereby obtaining enteromorpha protein. The protein extracted by the process is high in content and purity, and the process is simple and convenient, and is environment-friendly.

Description

technical field [0001] The invention belongs to the technical field of enteromorpha processing, in particular to a process for enzymatically extracting enteromorpha protein. Background technique [0002] Enteromorpha belongs to the Ulvaceae plant of the Chlorophyta Ulvae. There are mainly 5 species of Enteromorpha, Enteromorpha enterosum, Enteromorpha flatus, Enteromorpha enterosum, and Enteromorpha tubules. They grow in the intertidal zone and are rich in wild algae resources in my country. , especially widely distributed along the southeast coast. It has been edible and medicinal algae since ancient times. The protein content of Enteromorpha is 13.21%, the fat content is 1.04%, and the ash content is 21.87%. It is a high-protein, low-fat algae. Enteromorpha protein has comprehensive nutrition and contains 8 kinds of amino acids necessary for human body. The essential amino acids and non-essential amino acids are close to the amino acid pattern proposed by FAO / WHO. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00
Inventor 荆晓丽
Owner QINGDAO AIHUALONG BIOTECH