Nanogold-polypeptide biological probe and preparation and application method
A nano-biological probe and bio-probe technology are applied in the functionalization and application fields of nanomaterials to achieve the effects of simple operation and reduction of research costs.
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Embodiment 1
[0032] Take 100mL of 0.01% chloroauric acid aqueous solution, heat it to boiling, quickly and accurately add 1mL of 1% trisodium citrate aqueous solution under stirring, the light yellow chloroauric acid aqueous solution turns light gray within 2 minutes, continue to boil, and the color of the solution changes sequentially For light purple, purple. Continue to boil for 15 minutes, when the solution does not change any more, stop heating, continue stirring overnight, filter with a 0.22 μm filter membrane, and store at 4°C. The ultraviolet absorption of the solution was tested, and it was found that the highest peak was at 525nm, and the prepared nano gold particle diameter was 40nm.
[0033] Take 200 μL of prepared 40nm gold and add 20 μL of 0.1M NaOH solution, and the resulting solution has a pH value of 10. Add 20 μL of 25 μM polypeptide solution to this solution, mix thoroughly, and assemble at room temperature for 30 minutes. At the same time, nano-gold-PEG was prepared ...
Embodiment 2
[0036] Take 100mL of 0.01% chloroauric acid aqueous solution, heat it to boiling, quickly and accurately add 1mL of 1% trisodium citrate aqueous solution under stirring, the light yellow chloroauric acid aqueous solution turns light gray within 2 minutes, continue to boil, and the color of the solution changes sequentially For light purple, purple. Continue to boil for 15 minutes, when the solution does not change any more, stop heating, continue stirring overnight, filter with a 0.22 μm filter membrane, and store at 4°C. The ultraviolet absorption of the solution was tested, and it was found that the highest peak was at 525nm, and the prepared nano gold particle diameter was 40nm.
[0037] Take 200 μL of prepared 40nm gold and add 20 μL of 0.1M NaOH solution, and the resulting solution has a pH value of 10. Add 20 μL of 25 μM polypeptide solution to this solution, mix thoroughly, and assemble at room temperature for 30 minutes. At the same time, nano-gold-PEG was prepared ...
Embodiment 3
[0040]Take 100mL of 0.01% chloroauric acid aqueous solution, heat it to boiling, quickly and accurately add 2mL of 1% trisodium citrate aqueous solution under stirring, the light yellow chloroauric acid aqueous solution turns light gray within 2 minutes, continue to boil, and the color of the solution changes sequentially For light purple, wine red. Continue to boil for 15 minutes, when the solution does not change any more, stop heating, continue stirring overnight, filter with a 0.22 μm filter membrane, and store at 4°C. The ultraviolet absorption of the solution was tested, and it was found that the highest peak was at 518nm, and the particle diameter of the prepared nano-gold was 15nm.
[0041] Take 200 μL of the prepared 15nm gold and add 20 μL of 0.1 M NaOH solution, and the resulting solution has a pH value of 10. Add 20 μL of 25 μM polypeptide solution to this solution, mix thoroughly, and assemble at room temperature for 30 minutes. At the same time, nano-gold-PEG w...
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