Quality control method of sunset abelmoschus root medicinal material as well as extract and preparation of sunset abelmoschus root
A technology for the determination of hollyhock flowers and methods, which is applied in the direction of measuring devices, material separation, and analysis of materials, and can solve problems such as good stability, high detection sensitivity, and the inability to objectively and reasonably control the quality of medicinal materials and their preparations
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Embodiment 1
[0050] (1) Preparation of sample solution
[0051] Accurately weigh 0.1 g of hollyhock flower medicinal material, add 20 ml of 70% methanol, ultrasonically extract for 30 min, make up for weighing, filter, take the subsequent filtrate to pass through a 0.45 μm filter membrane, and inject 1 μL into the sample.
[0052] (2) Preparation of reference substance solution
[0053] Precisely weigh rutin, hyperoside, isoquercitrin, cottoncetin-8-O-glucuronide, myricetin, quercetin-3′-O-glucuronide, and quercetin reference substances Each appropriate amount was placed in a measuring bottle, and 70% methanol was used to prepare reference substance solutions with concentrations of 10 μg / mL, 235 μg / mL, 110 μg / mL, 250 μg / mL, 50 μg / mL, 101 μg / mL, and 16 μg / mL, respectively. Take different volumes of a single reference substance solution, mix and dilute to make a series of mixed reference substance solutions, and store them at 4°C for later use.
[0054] (3) Acquisition of liquid chromatogr...
Embodiment 2
[0072] (1) Preparation of sample solution
[0073] Accurately weigh 0.1 g of hollyhock flower extract, add 50 ml of methanol with a concentration of 90%, ultrasonically extract for 30 min, filter, take the subsequent filtrate to pass through a 0.45 μm filter membrane, and inject 10 μL of sample.
[0074] (2) Preparation of reference substance solution
[0075] Accurately weigh the appropriate amount of hyperoside, isoquercitrin, cottoncetin-8-O-glucuronide, myricetin, quercetin-3′-O-glucoside, rutin and quercetin reference substances , placed in a volumetric flask, with 90% methanol to prepare reference solutions with concentrations of 87 μg / mL, 401 μg / mL, 264 μg / mL, 462 μg / mL, 450 μg / mL, 751 μg / mL, and 103 μg / mL. Measure different volumes of a single reference substance solution, mix and dilute to make a series of concentration mixed reference substance solutions, and store them at 4°C for later use.
[0076] (3) Acquisition of liquid chromatogram
[0077] Get step (1) sam...
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