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Application of mechano-growth factor in preparation of serum-free cultured tolerant type mammal engineering cell

A technology of serum-free culture and growth factors, applied in the field of cell engineering and genetic engineering recombination, to achieve the effect of reducing requirements, improving tolerance and broad application prospects

Inactive Publication Date: 2013-04-10
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether MGF can be applied in the field of cell engineering to improve the tolerance of mammalian engineering cells to serum-free culture in large-scale fermentation environments has not been reported in domestic and foreign literature.

Method used

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  • Application of mechano-growth factor in preparation of serum-free cultured tolerant type mammal engineering cell
  • Application of mechano-growth factor in preparation of serum-free cultured tolerant type mammal engineering cell
  • Application of mechano-growth factor in preparation of serum-free cultured tolerant type mammal engineering cell

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Embodiment Construction

[0017] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0018] 1. Construction and identification of engineered mammalian cells stably expressing mouse MGF

[0019] The mouse MGF coding gene whose nucleotide sequence is shown as SEQ ID No.4 was synthesized. Follow ViraPower TM Lentiviral Directional TOPO ? According to the instructions of Expression Kit (Invitrogen), the following primers were synthesized: upstream primer F: 5'-caccatgggaccggagacgc-3'; downstream ...

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Abstract

The invention belongs to the cell engineering and gene engineering recombination technical filed, and relates to an application of a mechano-growth factor (MGF) in preparation of serum-free cultured tolerant type mammal engineering cell, the engineering cell of the MGF capable of being autonomously expressed can be constructed by transferring the MGF coding genes to the mammal cell, the engineering cell can substantially enhance the tolerance of serum-free culture by expressing the MGF, the fermentation condition requirement can be reduced, the culture cost is reduced, and the application of the mechano-growth factor (MGF) in preparation of serum-free cultured tolerant type mammal engineering cell is especially suitable for industrial large scale production of biological products, and has wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of cell engineering and genetic engineering recombination, and relates to the application of a new type of growth factor in improving engineered cells, in particular to the application of Mechano growth factor (MGF) in the preparation of serum-free culture tolerant mammalian engineered cells in the application. Background technique [0002] In the field of biotechnology, genetic engineering recombination technology is undoubtedly the most revolutionary technology. This technology enables humans to transform species, construct engineered bacteria, mammalian engineered cells, transgenic animals and plants, etc. through genetic recombination, so as to obtain the desired Biological products or more economically valuable biological varieties. For a long time, Escherichia coli expression system has been used for the production of recombinant proteins, but many important proteins cannot be actively expressed in Es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/12C07K14/65
Inventor 张兵兵鲜成玉王远亮徐洪记赵源
Owner CHONGQING UNIV