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Method for increasing plant biomass

一种生物量、植物的技术，应用在植物学设备和方法、生物化学设备和方法、植物再生等方向，能够解决昂贵、不容易接受等问题

Inactive Publication Date: 2013-04-10
BIOMASS BOOSTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, methods involving genetic manipulation of plants are usually expensive and not socially acceptable

Method used

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  • Method for increasing plant biomass
  • Method for increasing plant biomass
  • Method for increasing plant biomass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0168] Example 1: Increased plant biomass in carrot and tobacco plants

[0169] Materials and methods

[0170] Carrot (Daucus carota) and tobacco (Nicotiana tabacum) calli were provided by Carolina Biological Supply Company (Burlinton, NC, USA) and kept under sterile conditions in solid callus induction medium for carrot and tobacco, respectively ( Also obtained from Carolina Biological). Its specific components are available in the company's catalogue.

[0171] Individual calli were divided into small sections, and these small sections were weighed under sterile conditions, and then inoculated in fresh cells containing different concentrations of the synthetic peptide human adrenomedullin (AM) (Phoenix Pharmaceuticals, Burlingame, CA, USA). medium (solid callus induction medium was obtained from Carolina Biologicals). After 30 days of dark growth, the calli were weighed again and the growth rate was calculated as the quotient of final weight divided by initial weight. ...

Embodiment 2

[0177] Example 2: Increased algal biomass in microalgae of the genus Chlorella

[0178] Materials and methods

[0179] Two identical cultures (250 ml each) of Chlorella in Guillard F / 2 medium were prepared in two separate glass flasks [Guillard, R.R.L. 1975. Culture of phytoplankton for feeding marine invertebrates, pp26-60. In Smith W.L. and Chanley M.H (Eds.) Culture of Marine Invertebrate Animals, Plenum Press, New York, USA; Guillard, R.R.L. and Ryther, J.H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Can . J. Microbiol. 8:229-239]. 100 μl of the Guillard F / 2 medium was then added to the flask, and to another flask containing enough to reach 10 -8 The amount of the synthetic peptide human adrenomedullin (AM) (Phoenix Pharmaceuticals, Burlingame, CA, USA) at a final concentration of M in 100 μl of the Guillard F / 2 medium.

[0180] Continuously sparge the culture with 5% CO 2 air. The flasks were irradiated ...

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Abstract

The invention relates to the use of a peptide comprising a six-member ring created by the disulfide bond between two cysteines in the increase of biomass of a photosynthetic organism, for its application in the wood industry, in obtaining energy coming from renewable sources and in agriculture.

Description

technical field [0001] The present invention is comprised in the field of biotechnology, in particular, in the production of plant biomass. The present invention therefore relates to the use of peptides containing a six-membered ring generated by a disulfide bond between two cysteines for increasing plant biomass, which has applications in the wood industry, in obtaining energy from renewable resources, and in agriculture Applications. Background technique [0002] Cell growth in both plants and animals is regulated by a series of extracellular signals known as hormones or growth factors (Galiha et al. 2009, Semi Cell Dev Biol. 20:1149-1156), which act through specific membrane receptors Effect (Santner and Estelle2009, Nature459:1071-1078; De, I et al.2009, Nat Cell Biol11:1166-1173). These hormones can be synthesized within the plant or they can originate from outside organisms, as is the case with factors produced by rhizobia that promote the growth of their symbiotic p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H3/04C07K14/415C07K14/435C12N15/82A01N63/50
CPCC07K14/415C07K14/435C07K14/575C12N5/0025C12N15/8257C12N15/8261A01N65/08A01N65/44A01H3/04A01N63/50A01N63/10C12N15/82Y02A40/146C12N1/12
Inventor 阿尔弗雷多·马丁内斯拉米雷斯乔治·孔拉多·阿雷纳斯维达尔
Owner BIOMASS BOOSTER