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Production method for preparing highly unsaturated active rapeseed oil by biological enzyme method

A biological enzymatic and unsaturated technology, applied in the direction of fat oil/fat production, fat production, etc., can solve the problems of poor production safety, flammability and explosion, leached crude oil contains many non-oily substances, etc.

Inactive Publication Date: 2014-05-07
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pressing method mainly uses mechanical external force to squeeze the oil out of the oil. The product does not contain solvent, but the residual oil content of the cake is high, the oil extraction efficiency is low, and the power consumption is large; the leaching method uses a special organic solvent to extract the oil. It has high productivity, high oil yield, and is easy to realize large-scale production. However, most of the solvents used are flammable and explosive, and the production safety is poor. darker, lower quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 100g of peeled rapeseed, cook in boiling water (material-to-liquid ratio 1:3) for 5-10min, cool the boiled rapeseed to room temperature, and use a colloid mill (JMS-50 Langfang Guantong Machinery Co., Ltd.), with a material-to-liquid ratio of 1 : 4, refining for 3-5min. Put the ground slurry into the enzymolysis tank, adjust the pH to 4.5, control the enzymolysis temperature at 50°C, add 1% of the enzyme, and the ratio of pectinase NCB-PE40, Pectinex Ultra SP-L, and Viscozyme L is 1: 1:1, enzymatic hydrolysis for 3 hours, adjust the pH to 9, raise the temperature to 60°C, keep for 1 hour, add 2.4 L of 1% alkaline protease Alcalase, control the pH to 9, enzymatic hydrolysis for 2 hours, and centrifuge at 3500r / min for 15 minutes to obtain clear oil.

Embodiment 2

[0015] Take 100g of peeled rapeseed, cook in boiling water (material-to-liquid ratio 1:3) for 5-10min, cool the boiled rapeseed to room temperature, and use a colloid mill (JMS-50 Langfang Guantong Machinery Co., Ltd.), with a material-to-liquid ratio of 1 : 4, refining for 3-5min. Put the ground slurry into the enzymolysis tank, adjust the pH to 5, control the enzymolysis temperature at 55°C, add 2% of the enzyme, and the ratio of pectinase NCB-PE40, Pectinex Ultra SP-L, and Viscozyme L is 1: 1:2, enzymatic hydrolysis for 3 hours, adjust the pH to 10, raise the temperature to 60°C, keep for 1 hour, add 2% Alcalase 2.4L, control the pH to 10, enzymatic hydrolysis for 2 hours, centrifuge at 3500r / min for 15 minutes to obtain clear oil.

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Abstract

The invention develops a method for preparing highly unsaturated active rapeseed oil by a biological enzyme method and belongs to the technical field of food processing. The production method comprises the following processes: peeling rapeseeds, inactivating enzyme in boiling water, grinding, carrying out composite enzymatic hydrolysis and breaking wall (NCB-PE40, Peetinex Ultra SP-L, Viscozyme L), alkaline extraction, protease hydrolysis (2.4L of Alcalase) and centrifugally separating to obtain the highly unsaturated active rapeseed oil. The method for preparing the highly unsaturated active rapeseed oil by the biological enzyme method has the characteristics that firstly, through the biological enzyme method to break the wall, the release of the rapeseed oil is promoted, and the oil output is greater than 95%; secondly, through proper protease enzymatic hydrolysis and the centrifugal separating (at 2000-5000rpm), the rapeseed oil is obtained, and the clean oil rate is higher than 60%; and thirdly, in comparison with the conventional pressing and extracting methods, the method disclosed by the invention is simple and easy to implement, and green and environment-friendly; the product is free from any pollution and solvent residue; the produced rapeseed oil contains more than 85% of unsaturated fatty acid, and is high in nutrition value and strong in activity.

Description

1. Technical field [0001] The invention provides a production method for preparing highly unsaturated active rapeseed oil by a biological enzyme method, which belongs to the technical field of food processing. 2. Background technology [0002] Rapeseed is a cruciferous crop, with a planting area of ​​413 million mu in the world and an output of 51 million tons, ranking second in oil crops. my country's rapeseed planting area is 116 million mu, and the output is 11 million tons, accounting for 24% and 25% of the world respectively, ranking first in the world. Rapeseed is an important oil crop in my country, accounting for 50% of domestic oil crops, rapeseed oil consumption accounts for more than 35% of domestic edible vegetable oil, and occupies an important position in the domestic edible oil market. [0003] Industrial preparation of rapeseed oil mainly adopts pressing method and leaching method. The pressing method mainly uses mechanical external force to squeeze the oil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/00
Inventor 李春阳李跃张红城王帆
Owner JIANGSU ACAD OF AGRI SCI