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Kit for rapid quantitative detection of serum amyloid a and its preparation and application

A technology for quantitative detection and serum starch, which is applied in the field of clinical immunology detection, can solve the problems of high detection cost, achieve the effects of shortening the healing time, good application prospects, and reducing medical expenses

Active Publication Date: 2015-08-26
SHANGHAI UPPER BIO TECH PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the only domestic reagents approved by the State Food and Drug Administration (SFDA) for the detection of SAA in clinical specimens are latex-enhanced rate nephelometry on the supporting protein analyzer (SFDA (Join) Word 2012 No. 2403367) use
Due to the relatively high cost of detection and the need for special instruments, SAA detection has not been widely used in clinical practice.

Method used

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  • Kit for rapid quantitative detection of serum amyloid a and its preparation and application
  • Kit for rapid quantitative detection of serum amyloid a and its preparation and application
  • Kit for rapid quantitative detection of serum amyloid a and its preparation and application

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Embodiment 1

[0037] 1. Preparation of freeze-dried anti-SAA immune colloidal gold and reaction plate: according to the method introduced by Lu Shengkai et al. , 1990) to prepare colloidal gold and anti-SAA immune colloidal gold conjugates. (Measure 2500ml of double distilled water into a 5000ml Erlenmeyer flask, heat it together with an appropriate amount of glass beads until boiling, add 1% trisodium citrate solution in the boiling state (the amount to be added depends on the small test), and then add 1% chlorine The gold solution was boiled for 10-20 minutes, cooled at room temperature, and the liquid was clear and transparent wine red. Into the above-mentioned colloidal gold solution that had been cooled to room temperature, the K 2 CO 3 Adjust the pH of the solution, mix well, then add SAA monoclonal antibody according to the initial test ratio, mix well, shake several times in the middle, and then add half the amount of K 2 CO 3 Solution, and 1% Carbowax solution, so that the final...

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Abstract

The invention relates to a kit for quickly and quantificationally detecting serum amyloid A (SAA) by using an immunogold method. The kit includes lyophilized anti-SAA immunogold, a reaction plate, a sample diluent, a lyophilized colloidal gold complex solution, a scrubbing solution and a sealing solution. The preparation method comprises the following steps: (1) firstly preparing a conjugate of the colloidal gold and anti-SAA immunogold, and preparing lyophilized anti-SAA immunogold; and (2) preparing the sample diluent, the lyophilized colloidal gold complex solution, the scrubbing solution and the sealing solution according to the proportion, preparing the reaction plate, and assembling the prepared objects to a finished product to obtain the kit. By using the kit provided by the invention, a doctor can obtain an SAA measurement result on the spot when a person is ill so as to quickly judge the patient's condition, so that more targeted treatment can be effectively taken on the patient, healing time is shortened and medical cost is reduced. The kit provided by the invention has a good application prospect.

Description

technical field [0001] The invention belongs to the field of clinical immunology detection, in particular to a kit for rapid quantitative detection of serum amyloid A and its preparation and application. Background technique [0002] Serum amyloid A (serum amyloidA, SAA) is a group of pleomorphic proteins encoded by the same cluster of genes, which is the precursor of amyloid A deposited in amyloid tissue and belongs to the acute phase response protein (Uhlar CM, Whitehead AS. Serum amyloid A, the major vertebra acute-phase reactant [J]. Eur J Biochem, 1999, 265(2): 501-523). SAA can increase rapidly in the acute phase of inflammation or infection (48-72h), and rapidly decrease in the recovery phase of the disease (Urieli SS, Linke RP, Matzner Y. Expression and function of serum amyloid A, a majoracute-phase protein, in normal and disease states[J].Hematology, 2000,7(1):64–69), for example in virus infection (R.Shainkin-Kestenbaum, S.Zimlichman, Y. Winkoff, M.Pras, C.Chaimo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/531
Inventor 杨晶沈旭辉翟小杰
Owner SHANGHAI UPPER BIO TECH PHARMA
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