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Method for rapid and vegetative propagation of crowtoe

A technology of asexual reproduction and L. japonicus, applied in the biological field, can solve problems such as long reproduction time

Active Publication Date: 2015-04-08
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem existing in the asexual reproduction technology of japonicus japonicus is that the reproduction time is long, and it usually takes 70 to 90 days to obtain a generation of asexual propagules

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: a kind of method of rapid, asexual propagation japonicus japonicus

[0013] Proceed as follows:

[0014] 1. Disinfection of explants and induction of adventitious buds

[0015] Select lotus root seedlings that have germinated for 6-8 days and have two cotyledons fully expanded, cut off the cotyledons from the base of the cotyledons, soak them in 70% alcohol for 30 seconds, and then wash them with 0.1% HgCl 2 The solution was sterilized for 3 minutes, washed with sterile water for more than 3 times, and inoculated on the adventitious bud induction medium. Adventitious bud induction medium is: take MS medium as the basic medium, add 0.5mg of 6-benzyl adenine per liter of basic medium, add 3% sucrose and 0.7% agar by weight percentage, and adjust the pH to 5.8 . The culture conditions are 25±1°C, 16 hours of light per day, and the light intensity is 30umol -2 the s -1 . Adventitious buds appear after 10 days of cultivation; after 20 days, the adventit...

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Abstract

The invention relates to a method for rapid and vegetative propagation of crowtoe. The adopted technical scheme is as follows: the method comprises the steps of sterilizing cotyledon of the crowtoe; then inoculating cotyledon of the crowtoe onto an adventitious bud induction medium, wherein the culture condition is that the temperature is 25 plus or minus 1 DEG C, the illumination time is 16 hours each day, and the light intensity is 30micromol-2s-1, and cultivating till the adventitious bud sprouts; when the adventitious bud is 2-3cm, cutting from the root, and transferring the root into a rooting culture medium; when the root of the rooting seedling is 2-3cm, opening a bottle neck for hardening seedling, and then transplanting into a mixed matrix. According to the method, a plant tissue culture method is adopted, leaves are taken as material, the formation of the adventitious bud is directly induced without the callus step, and the adventitious bud can be induced to form a complete root system in 20 days through a rooting manner. The generation of vegetative propagation offspring can be obtained in 40-50 days. The method provides a feasible manner for the rapid propagation of the crowtoe.

Description

technical field [0001] The invention relates to a method for rapid and asexual propagation of japonicus japonicus, belonging to the field of biotechnology. Background technique [0002] japonicus ( Lotus corniculatus L.), also known as hornflower, five-leaf clover and bird's-foot grass, is a perennial herbaceous plant of the genus Libido, one of the world-renowned fine legume forages, and is also a common garden ornamental plant. Lotus japonicus is native to the humid regions of Eurasia, and is currently planted in Europe, America, Australia and South Asia. It is distributed in South China, Southwest my country, Northwest China, North China and Northeast China. It is a plant with great potential in warm and humid regions of my country. Good pasture. As a high-quality leguminous forage, japonicus japonicus has the advantages of cold resistance, waterlogging resistance, barren resistance, trampling resistance, and drought resistance. It can grow well even on barren hillsides...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 韩阳娄虹卢福荣段雅楠
Owner LIAONING UNIVERSITY