Pentapeptide, polypeptide, cyclic peptide derivative, compound and its medicine and application
A technology of derivatives and compounds, applied in the field of immunosuppressive drugs, can solve the problems of no single compound and unreported research
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Embodiment 1
[0041] Example 1 The inhibitory effect of different concentrations of TIPP on the proliferation of mouse spleen lymphocytes
[0042] Male Balb / c mice at 6 to 8 weeks were sacrificed by cervical dislocation, soaked in 75% alcohol for 5 minutes, and the mice were taken out and placed in a sterile dish with the left side facing up. A small incision was made in the middle of the left ventral side of the mouse, the skin was torn off, and the abdominal wall was exposed. A red strip-shaped spleen was visible. Lift the peritoneum on the lower side of the spleen, cut it open and turn it up to expose the spleen, lift the spleen with tweezers, separate the connective tissue under the spleen with ophthalmic scissors, and take out the spleen. Put it into a petri dish and wash it twice with 5 mL of Hank's solution. Place the spleen on a stainless steel mesh (200 mesh), press the spleen gently with a syringe needle core, and rinse with 7-8mL of Hank's solution, collect the cell suspension i...
Embodiment 2
[0044] The toxicity of embodiment 2 different concentration medicines to mouse spleen lymphocyte
[0045] Male Balb / c mice at 6 to 8 weeks were sacrificed by cervical dislocation, soaked in 75% alcohol for 5 minutes, and the mice were taken out and placed in a sterile dish with the left side facing up. A small incision was made in the middle of the left ventral side of the mouse, the skin was torn off, and the abdominal wall was exposed. A red strip-shaped spleen was visible. Lift the peritoneum on the lower side of the spleen, cut it open and turn it up to expose the spleen, lift the spleen with tweezers, separate the connective tissue under the spleen with ophthalmic scissors, and take out the spleen. Put it into a petri dish and wash it twice with 5 mL of Hank's solution. Place the spleen on a stainless steel mesh (200 mesh), press the spleen gently with a syringe needle core, and rinse with 7-8mL of Hank's solution, collect the cell suspension in a 10mL sterile centrifuge...
Embodiment 3
[0047] Example 3 Inhibitory Effect of Different Concentrations of TIPP on RAW264.7 Cell Proliferation
[0048] Take RAW264.7 cells in the logarithmic growth phase cultured in DMEM medium (10% FBS), and adjust the cell density to 2×10 4 cells / mL, seeded on a 96-well plate with a volume of 100 μL per well, and placed at 37°C, 5% CO 2 The cells were cultured for 2 h under conditions to make the cells adhere to the wall, and different concentrations of TIPP were added (final concentrations were 25 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL, and 5 replicate wells were set for each concentration). Dexamethasone (Dex, with a final concentration of 5 μg / mL) was used as a positive control, and culture was continued for 48 hours. Add 20 μL of 5 mg / mLMTT to each well, and incubate at 37°C, 5% CO 2 After continuing to culture for 4 hours under the same conditions, centrifuge at 3000r / m for 10min, remove the supernatant carefully with a 1mL syringe, add 150μL of DMSO to each well, ...
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