Production of saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation used for feed

The technology of Lactobacillus acidophilus and Saccharomyces cerevisiae is applied in the production field of compound microbial preparations for feed of Saccharomyces cerevisiae and Lactobacillus acidophilus, which can solve the problems of excessive use of antibiotics and low protein utilization rate, so as to enhance immunity, The effect of simple and universal cultivation raw materials and simple production process

Active Publication Date: 2013-06-26
辽宁威兰生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in livestock and poultry farming and aquaculture, there are two major problems faced by producers and consumers: 1. The utilization rate of protein is not high; 2. Excessive use of antibiotics

Method used

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  • Production of saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation used for feed
  • Production of saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation used for feed
  • Production of saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation used for feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Saccharomyces cerevisiae (protease, amino acid, vitamin, biotin) single colony purification, solid primary seed culture, liquid secondary fermentation culture, inspection and determination of bacterial count, the specific operation steps are as follows:

[0057] 1) Isolation and optimization of single colonies: apply activated Saccharomyces cerevisiae slant strains on a culture dish of newly configured yeast culture medium, and culture to obtain Saccharomyces cerevisiae single colony seeds.

[0058] Saccharomyces cerevisiae single colony purification and optimal medium composition are: 20% potato (potato) extract 1000mL, sucrose 20g, agar 18g, pH6.8; Saccharomyces cerevisiae primary solid seed medium can also be used.

[0059] 2) solid first-level seed culture: the Saccharomyces cerevisiae single colony seed obtained in the previous step 1) is inoculated into the yeast first-level solid seed medium, and the solid seed culture bottle is a 200ml eggplant-shaped flat bottle...

Embodiment 2

[0082] Lactobacillus acidophilus, (producing lactic acid, acetic acid and various organic acids, lactobacillus, peptide antibiotics, protease, vitamins, etc.) single colony purification and optimization, solid primary seed cultivation, liquid secondary fermentation cultivation The operation steps are as follows:

[0083] 1) Purification and optimization of a single colony: apply the activated Lactobacillus acidophilus slant strain on a culture dish of a newly prepared lactic acid bacteria medium, and culture to obtain a single colony seed of Lactobacillus acidophilus.

[0084] 2) solid first-level seed culture: the Lactobacillus acidophilus single colony seed obtained in the previous step 1) is inoculated into the first-level solid seed medium of Lactobacillus acidophilus, and the solid seed culture bottle is a 200ml eggplant-shaped flat bottle. Cultivate to obtain first-grade solid seeds of Lactobacillus acidophilus;

[0085] 3) Liquid secondary fermentation culture: the pro...

Embodiment 3

[0106] The compound microbial preparation for feed of Saccharomyces cerevisiae and Lactobacillus acidophilus was tested in young ginseng, and the effect of the compound microbial preparation for feed of Saccharomyces cerevisiae and Lactobacillus acidophilus on the weight gain of sea cucumbers is shown in Table 7;

[0107] Table 7. The effect of the compound microbial preparation of Saccharomyces cerevisiae and Lactobacillus acidophilus on the weight gain of sea cucumbers

[0108]

[0109] *Test period: March 1, 2011 to March 15, 2011

[0110] *Water body: length × width × height = 1m × 1m × 1m = 1m 3 (cubic meter water body)

[0111] *Test group 1 and test group 2 used 5 milliliters of compound microbial preparation for each water body every day;

[0112] The compound microbial preparation is formed by mixing the following two kinds of mother liquors in the same volume ratio of 1:1; the two kinds of mother liquors are respectively:

[0113] The secondary liquid fermentat...

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Abstract

The invention relates to a microbe preparation, and concretely relates to a production method of a saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation used for feed, the production method is characterized in that concentrated saccharomyces cerevisiae mother liquor containing saccharomyces cerevisiae greater than or equal to 50 hundreds million / ml and the concentrated lactobacillus acidophilus mother liquor with high density containing lactobacillus acidophilus greater than or equal to 100 hundreds million / ml are mixed according to 1:1 with the volume ratio, and the composite microbe preparation product containing saccharomyces cerevisiae greater than or equal to 50 hundreds million / ml and containing lactobacillus acidophilus greater than or equal to 100 hundreds million / ml. The saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation takes glucose, cane sugar, cane sugar honey and corn steep liquor as main fermentation raw materials, the fermental cultivation can be carried out at 30-34 DEG C, the product bacteria number is high, the quality is good, pH value is between 5.0-6.6, and the quality guarantee period is 18 monthes. The saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation used for feed has simple production technology and good product application effect, can purify the culture environment, and can be used in the culture industries such as aquatic product and livestock and poultry.

Description

Background technique [0001] Saccharomyces cerevisiae, also known as baker's yeast or budding yeast, is the most widely related yeast to humans. Traditionally used to make bread and wine, and used as eukaryotic model organisms in modern molecular and cell biology. The cell shape of Saccharomyces cerevisiae is spherical or ovoid, with a diameter of 510 μm. The mode of reproduction is budding, and its carbon and nitrogen sources are widely used, including glucose, maltose, sucrose, galactose, arabinose, mannitol, succinic acid, citric acid, xylose, sorbitol, fructose, ethanol and glycerin; Rhamnose, starch, cellobiose, trehalose, ribitol, inositol, erythritol, melibiose cannot be utilized. [0002] Lactobacillus acidophilus (Lactobacillus acidophilus) belongs to the Lactobacillus genus, is a Gram-positive bacillus, and the end of the rod is round, and is mainly present in the small intestine. Can secrete lactic acid, acetic acid and peptide antibiotics. Lactobacillus acidophi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18A23K1/16C12R1/865C12R1/23
Inventor 薛德林刘志国李威朱颖
Owner 辽宁威兰生物技术有限责任公司
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