Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids

Abstract

The invention relates to a method for culturing crops and breeding hybrids, and particularly relates to a method for culturing kohlrabi and breeding hybrids of kohlrabi, which belongs to the technical field of plant seed production. The method comprises the following steps of: crossing the male sterility line of wild cabbage with the inbred line (namely the maintenance line) of kohlrabi with inbreeding of multiple generations for purification and stable traits to obtain an intermediate, and then carrying out continuous backcross on the maintenance line of kohlrabi to obtain the male sterility line of kohlrabi through transformation; and crossing the male sterility line of kohlrabi as a female parent with the inbred line of kohlrabi with inbreeding of multiple generations for purification and stable traits as a male parent, thus obtaining the high-quality variety of kohlrabi with high purity. By utilizing the same spice of plants to transform sterility sources, the method has the beneficial effects that the seed production of the sterility line is simple, the labor and the cost are reduced, the sterility rate of the sterility line reaches to 100% and the transformation path of the sterility line material of kohlrabi is added; and when new varieties are cultured by the male sterility line, the purity of prepared hybrids reaches to 100%, and the purity of seeds is improved.

Description

technical field [0001] The invention relates to a method for cultivating and selecting hybrids with crop breeding materials, in particular to a method for cultivating and selecting hybrids with kohlrabi breeding materials, and belongs to the technical field of plant seed production. Background technique [0002] Kohlrabi, also known as kohlrabi (Brassica olerecea L.var.caulorapa), is a Brassicaceae, Brassica, cabbage vegetable, native to the European Mediterranean to the North Sea coast. Kohlrabi has a very high nutritional value. Its carbohydrate and nitrogen content is twice that of cabbage, and its vitamins are 0.5-1 times more than that of cabbage. The vitamin C it contains is equivalent to that of citrus. The cultivated area in South China and Sichuan is relatively large, and the cultivated area in the middle and lower reaches of the Yangtze River has increased year by year in recent years. In China, the research on the breeding of new varieties of kohlrabi started rel...

AI Technical Summary

Problems solved by technology

In China, the research on the breeding of new varieties of kohlrabi started relatively late, and there is a big gap between the breeding level and that of major vegetables such as cabbage. Incompatibility lines, using artificial pollination to produce parents, its disadvantages are: the cost of parental seed production is high, and the purity of hybrids is difficult to guarantee

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