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Pressurizable cartridge for polymerase chain reactions

A reaction flask, pressure technology, applied in specific-purpose bioreactor/fermenter, laboratory container, bioreactor/fermenter combination, etc., can solve problems such as blockage

Active Publication Date: 2013-07-17
B·L·韦特 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, air bubbles can at least partially block and / or distort and / or refract the optical signal, and in doing so, degrade signal quality and / or introduce noise received at the optical detector
Prior art solutions attempt to solve this problem, often with limited success, by using very precise and therefore very expensive thermal controllers to try and prevent boiling from occurring

Method used

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  • Pressurizable cartridge for polymerase chain reactions
  • Pressurizable cartridge for polymerase chain reactions
  • Pressurizable cartridge for polymerase chain reactions

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Embodiment Construction

[0035] The polymerase chain reaction is an important tool used as a precursor for activities such as identifying small amounts of specific genetic material in a sample, determining how much genetic material is present in a sample, or producing sufficient amounts of genetic material for various applications. genetic material.

[0036] Traditional circulating thermostats take many forms. The most common thermocycler uses multiple vials placed in a large solid thermal block. Each vial is manually loaded with the sample DNA desired to be amplified—hereinafter also sometimes referred to as "template DNA"—and the chemical components necessary for the polymerase chain reaction. The steps of the PCR process are performed in laboratories by skilled technicians.

[0037] refer to figure 1 , the present invention provides a sample processing module 20 that simplifies the PCR process, aspects of which are filed on December 17, 2007 and entitled "PCR Sample Processing Module (PCR Sample...

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Abstract

Methods and apparatus for use in connection with the performance of the polymerase chain reaction are provided. An exemplary sample processing module is described that includes a sample assembly and a PCR assembly, the sample processing module being configured to hold the sample therein at a pressure higher than ambient pressure. A sample is added to the sample assembly at the time of use, which is then connected to the PCR assembly. Embodiments of the cartridge include a flow restriction device that enables or aids in creating a higher pressure within the reaction vial. The sample is introduced into a PCR reaction vial, which contains all of the constituents of a PCR reaction mixture that are necessary to process the sample and provide amplified DNA of interest, if that DNA was present in the sample.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application No. 61 / 369,925, filed August 2, 2010, the entirety of which is hereby incorporated by reference for all purposes. technical field [0003] The present invention relates to the field of thermocyclers used in the practice of polymerase chain reaction (PCR). Background technique [0004] An important tool in the field of molecular biology is the process known as the "polymerase chain reaction" (PCR). PCR produces large quantities of genetic material from a small sample of genetic material. [0005] The PCR process is carried out in a small reaction vial containing the following DNA replication components: the DNA to be replicated and a PCR reaction reagent usually carried in a carrier fluid, which includes the four nucleotides assembled to form the DNA, the two Different types of synthetic DNA called "primers" (one primer for each complementary strand of ...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/38C12Q1/68C12N15/10
CPCB01L3/5082B01L2400/0478B01L3/5029B01L2200/0684B01L2300/0681G01N27/44791B01L7/525B01L2300/123B01L2300/022B01L7/52B01L3/502723C12Q1/686B01L3/50273B01L3/502715
Inventor B·L·韦特W·D·比克莫尔D·K·西廷
Owner B·L·韦特