Bacillus amyloliquefaciens and application thereof

A technology for dissolving starch spores and bacilli, applied in application, bacteria, fungicides, etc., can solve the problems of yield, quality, benefit impact, high labor cost, and orchard yield reduction, etc., and achieve good application prospects, large spore production, and growth. fast effect

Inactive Publication Date: 2013-07-24
青岛秾贝尔生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the disease spreads, it will have a great impact on yield, quality, and benefit, causing many orchards to reduce production or even face the danger of destroying the orchard.
For the pr

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0020] Example 1

[0021] 1. Isolation and purification of B.amyloliquefaciens strain RCD-1

[0022] The B. amyloliquefaciens RCD-1 of the present invention is obtained from the soil by the dilution plate method and the plate streak method. The separation method is:

[0023] (1) Separation of Bacillus: The soil samples were collected on the beaches along the beach in the Weibei area of ​​Weifang City, Shandong Province. The 5-point sampling method was used to collect appropriate amounts of soil around the plot and the central depth within the range of 10-20cm, etc. Mix well. Indicate the location, time and person of collection. Weigh 1g soil sample in 100mL sterile water, place it in a shaker at 30℃ and shake at 150rpm for 10min, then place it in a 60℃ water bath and incubate for 30min, take 100μL of 10 -2 , 10 -3 , 10 -4 The dilution was spread on the beef extract peptone medium plate, and each gradient was spread in three parallels. After incubating at 30°C for 2 days, the differ...

Example Embodiment

[0032] Example 2

[0033] 1. Fermentation process of Bacillus amyloliquefaciens strain RCD-1

[0034] Beef extract peptone liquid medium, the formula is: tryptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1000mL, pH adjusted to 7.0-7.2.

[0035] A large amount of liquid fermentation medium formula (mass percentage content): Soy flour 2.5%, corn flour 3.5%, NaCl 0.1%, CaCO 3 0.2%, the rest is water.

[0036] Mass solid fermentation process of Bacillus amyloliquefaciens strain RCD-1:

[0037] (1) Transplant the spores of Bacillus amyloliquefaciens strain RCD-1 into beef extract peptone liquid medium, shake culture at 28-30℃ for 2-3 days to obtain seed liquid;

[0038] (2) Inoculate the seed liquid into a large amount of fermentation medium at a ratio of 3.0% (volume ratio), the cultivation temperature is 28-30℃, the cultivation time is 30h, and the ventilation ratio is 1:0.9. After the fermentation is completed, the fermentation broth is obtained, and the bacterial count o...

Example Embodiment

[0040] Example 3 Verification of indoor effect of Bacillus amyloliquefaciens strain RCD-1 on grape white rot bacteria

[0041] 1. Experimental method

[0042] Using the plate confrontation culture method, the tested grape white rot bacteria cake (9mm) was moved to the center of the plate, and the Bacillus amyloliquefaciens strain RCD-1 was inoculated at 2 cm around the plate, and cultured in a 27°C biochemical incubator. Repeat 3 for each treatment Times. When the control hyphae grows over the plate, the cross method is used to measure the colony diameter and the diameter of the inhibition zone, and calculate the average value and relative inhibition rate.

[0043] 2. Results

[0044] It can be seen from Table 1 that the Bacillus amyloliquefaciens strain RCD-1 has a strong inhibitory effect on the growth of white rot fungus hyphae. The diameter of the inhibition zone is 15.16mm, and the inhibition rate of hypha growth is 83.17%.

[0045] Table 1. Indoor antibacterial activity of Bacil...

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Abstract

The invention discloses bacillus amyloliquefaciens and application thereof, and belongs to the field of biological control of plant diseases. The strain is bacillus amyloliquefaciens RCD-1, and the collection number is CGMCC No.7502. The invention further discloses a biological control preparation containing the strain and a preparation method of the biological control preparation. The bacillus amyloliquefaciens strain provided by the invention has the characteristics of high growth speed, high spore yield, wide effect spectrum and strong stress resistance, and can be fast colonized in quantity at the plant roots, so good application prospect is achieved. The biological control preparation prepared from the bacillus amyloliquefaciens not only can effectively control the soil-borne disease of the plant, but also can increase the crop output, and is a biological control preparation with great application prospect. The microbial preparation can serve as biopesticide or biological fertilizer for controlling various soil-borne plant diseases, especially grape white rot.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a strain of Bacillus amyloliquefaciens capable of efficiently preventing and treating plant diseases and its use, and a biological control agent prepared by using the Bacillus amyloliquefaciens. Background technique [0002] Grapes are deciduous vines, known as the "God of Fruits", and are one of the oldest plants in the world. Grapes are native to Europe, West Asia and North Africa. The main producing areas in my country are Xinjiang, Gansu, Shanxi, Hebei, Shandong and other places. Grapes can be eaten directly as fruit or used to make wine. Eating grapes has anti-virus and anti-bacterial effects, anti-cancer and anti-cancer, reducing stomach acid and gallbladder, anti-atherosclerosis, tonifying and stimulating brain nerves, diuretic, swelling and anti-abortion; the brewed wine can nourish qi and blood, nourish yin and promote ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 李鹏牛赡光王清海李泉涌刘幸红李红梅
Owner 青岛秾贝尔生物工程有限公司
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