Unlock instant, AI-driven research and patent intelligence for your innovation.

System and process for biopolymer chromatography

A biopolymer, chromatography system technology, applied in the field of continuous or semi-continuous, large-scale chromatography separation, can solve the problems of complex repeatable results and achieve the effect of continuous operation

Active Publication Date: 2013-07-24
CYTIVA SWEDEN AB
View PDF6 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, packing a column with chromatographic media to obtain reproducible results is complex

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • System and process for biopolymer chromatography
  • System and process for biopolymer chromatography
  • System and process for biopolymer chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] In one aspect illustrated by Figures 1-6, the present invention discloses a chromatographic system (1) for the separation of biopolymers comprising at least one feed tank 3, at least one storage tank 4; 4a, 4b, 4c, At least one elution buffer tank 5, at least one elution tank 6, at least two packed bed chromatography columns 7, 8 and for each packed bed chromatography column at least one pump 10 and at least one outlet detector 11, all connected to Each of the packed bed chromatography columns, wherein the feed tank, one or more storage tanks, the elution buffer tank and the elution tank are each connected to the packed bed chromatography column via a system of valves 12 . As defined above, one or more storage tanks are connected to at least one inlet port 13 of the column 7 , 8 and to at least one outlet port 14 of the column 7 , 8 , eg via a system of valves 12 . That is, at least two packed bed chromatography columns are provided, each having an inlet port 13 and an ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A chromatography system (1 ) for separation of a biopolymer is described, comprising at least one feed tank (3), at least one hold tank (4; 4a, 4b, 4c), at least one elution buffer tank (5), at least one eluate tank (6), at least two packed bed chromatography columns (7,8) and for each packed bed chromatography column (7,8) at least one pump 810) and at least one outlet detector (11) both connected to said each packed bed chromatography column (7,8), wherein the feed tank, the hold tank(s), the elution buffer tank and the eluate tank are each connected to the packed bed chromatography columns via a system of valves (12), and wherein said hold tank(s) is / are connected to at least one inlet end (13) of a column (7,8) and at least one outlet end (14) of a column (7,8).

Description

technical field [0001] The present invention relates to chromatographic separations and in particular to large scale chromatographic separations of biopolymers such as monoclonal antibodies. More particularly, it relates to chromatography systems with storage tanks and to continuous or semi-continuous methods of operating such systems. Background technique [0002] In the field of biopharmaceuticals, new advances in genetic engineering and cell culture techniques have enabled expression levels to be higher than before, placing a considerable burden on downstream purification, especially the capture step. Although the introduction of new chromatographic resins significantly improves the efficiency of conventional fixed-bed chromatography-based processes, additional gains can be realized by operating in a continuous mode. The latter is especially attractive when employing continuous bioreactors such as those operating in perfusion mode. [0003] A typical process for downstr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/18G01N30/44G01N30/46
CPCB01D15/3847G01N2030/8831B01D15/242B01D15/3809B01D15/1871G01N30/466G01N30/461B01D15/18G01N30/44G01N30/42G01N30/465
Inventor M.哈尔K.拉基
Owner CYTIVA SWEDEN AB