Artificial cultivating method for chestnut mycorrhiza fungi

Abstract

The invention discloses an artificial cultivating method for chestnut mycorrhiza fungi and belongs to the field of mycorrhiza fungi artificial cultivating. The artificial cultivating method for the chestnut mycorrhiza fungi includes the following steps: (1) taking a chestnut mycorrhiza fungi fruiting body, cutting the chestnut mycorrhiza fungi fruiting body into two pieces, and collecting intersecting parts of fungus caps and fungus stipes to obtain fungus context issue blocks; (2) putting the fungus context issue blocks in an inclined plane culture medium, culturing the fungus context issue blocks under the temperature of 25-28 DEG C until mycelia are germinated by the fungus context issue blocks, then the mycelia are cultivated under the temperature of 25-28 DEG C until the mycelia grow to cover the inclined plane after the mycelia is through tube turning to obtain pure mycelia; (3) inoculating the pure mycelia to an inoculating hole of an enlarged cultivating material, and cultivating the pure mycelia under the temperature of 25-28 DEG C until the pure mycelia grow all over a cultivating container to obtain the chestnut mycorrhiza fungi. The artificial cultivating method for the chestnut mycorrhiza fungi is simple in operation, strains cultivated and obtained through the artificial cultivating method for the chestnut mycorrhiza fungi is inoculated to chestnut seedlings, a mycorrhiza impregnating rate can reach 50.3% to the maximum degree, and an average impregnating rate is 46.5%.

Description

Technical field [0001] The invention belongs to the field of artificial cultivation of mycorrhizal fungi, and specifically relates to an artificial cultivation method of chestnut mycorrhizal fungi. Background technique [0002] Mycorrhiza is the most common symbiosis phenomenon of soil microbe and plant compatibility. The formation of ectomycorrhiza makes trees rely on the help of mycorrhizal fungi to increase the absorption capacity of soil moisture and nutrients. The organic phosphorus decomposing enzyme secreted by the ectomycorrhizal fungus can decompose and absorb the organic phosphorus in the litter and soil organic matter and directly transmit it to the trees for use, thereby having the effects of drought resistance, growth promotion and disease prevention. After the ectomycorrhizal fungi infect the roots of the host plant, on the one hand, the Harty's net and mycelium are formed, and on the other hand, the hyphae extend outward to form a dense hyphal network and even form...

AI Technical Summary

Problems solved by technology

In the conventional grafting of chestnut seedlings, due to the lack of mycorrhizal fungi that can co-exist with the root system of chestnut in the seedbed or culture medium, the conventional grafted seedlings transplanted to the field have low survival rate, slow growth and development, and after 2 to 3 years There will also be death of large trees

[0005] However, the symbiotic relationship between chestnut roots and ectomycorrhizal fungi is very complicated, and it is difficult to isolate and cultivate many mycorrhizal fungi.

There have been successful reports on the mycorrhization of chestnuts, but the inoculated bacteria are all foreign strains, and the inoculation effect is very unsatisfactory.

Although Qin Ling and others also successfully collected a variety of fungal fruiting bodies from the soil in chestnut-producing areas, they did not isolate the strains that can be successfully grafted back.