Pet food compositions for inducing satiety response
A pet food and composition technology, applied in the field of companion animals
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[0043] Example 1 - Screening
[0044] Affymetrix gene chip expression analysis
[0045] Gene expression analysis was performed using Affymetrix Canine 1 and Canine 2 GeneChip™ arrays commercially available from Affymetrix, Inc., Santa Clara, CA 95051. Total RNA was reverse transcribed into cDNA. cDNA is used to generate cRNA, which is fragmented and used as probes for gene chip hybridization. The gene chip was washed, and the hybridization signal was measured with an Affymetrix laser scanner. Hybridization data were then validated and normalized for further analysis.
[0046] Materials: Affymetrix offers most reagents and kits. Obtain other reagents listed in the Affymetrix handbook but not in the kit separately. For details, please refer to the Gene Chip Expression Analysis Technical Manual (701021 Rev.4).
[0047] Equipment: Eppendorf microcentrifuge, 1.5 mL DNase- and RNase-free sterile microcentrifuge tubes, 50 mL DNase- and RNase-free sterile disposable polypropyl...
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