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Analyzing method for insect cuticular hydrocarbon compound components

A hydrocarbon and insect technology, applied in the field of plant protection, can solve the problems of affecting the experimental results, unable to analyze the species, escaping, and possibly extracting the substances in the body, etc.

Inactive Publication Date: 2015-04-29
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Small molecule hydrocarbons can be separated using this technique, but larger molecular weight species cannot be analyzed because they cannot be extracted in sufficient quantities to be detected by GC-MS
The pheromone substances with larger molecular weight are closely combined with the body wall. When directly extracting with organic solvents, if the extraction time is too short, the hydrocarbons in the deeper layers of the insect body surface cannot be fully extracted due to the water blockage of the body wall; If the time is too long, since the substances in the insect body escape through the mouthparts or anus, the substances in the body may also be extracted, both of which will affect the experimental results

Method used

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  • Analyzing method for insect cuticular hydrocarbon compound components
  • Analyzing method for insect cuticular hydrocarbon compound components
  • Analyzing method for insect cuticular hydrocarbon compound components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Materials and Methods

[0029] Material:

[0030] The tested adult Lygus gnats came from the East Experimental Farm of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences; the specific models of the instruments used in the experiment are as follows.

[0031] method:

[0032] anaesthetization

[0033] Put 20 adult Lygus gnats into two airtight containers respectively, and use carbon dioxide to anesthetize for 120 seconds.

[0034] Freeze drying

[0035] Collect the anesthetized Lygus lucidum in one of the airtight containers, use liquid nitrogen for quick freezing, and immediately put it into a freeze dryer (Christ Alpha1-2LD, Germany) for freeze drying. The freeze drying conditions are cold trap temperature -64 ° C, vacuum degree 0.01 Below mbar, the freeze-drying time is 12h.

[0036] extraction

[0037] Put the freeze-dried Lygus viridans into a chromatographic bottle, add 5mL of dichloromethane for extraction, after 2 hours of extracti...

Embodiment 2

[0047] Materials and Methods

[0048] Material:

[0049] The tested adult Lygus gnats came from the East Experimental Farm of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences; the specific models of the instruments used in the experiment are as follows.

[0050] method:

[0051] anaesthetization

[0052] Put 20 adult Lygus females into two airtight containers respectively, and use carbon dioxide to anesthetize for 180 seconds.

[0053] Freeze drying

[0054] Collect the anesthetized Lygus lucidum in one of the closed containers, use liquid nitrogen for quick freezing, and immediately put it into a freeze dryer (Christ Alpha1-2LD, Germany) for freeze-drying. The freeze-drying conditions are cold trap temperature -84 ° C, vacuum degree 0.01 Below mbar, the freeze-drying time is 24h.

[0055] extraction

[0056] Put the freeze-dried Lygus viridans into a chromatographic bottle, add 5mL of n-hexane for extraction, after extraction for 6 hours, dr...

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PUM

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Abstract

The invention provides an analyzing method for insect cuticular hydrocarbon compound components. The method comprises the following steps of: freezing and drying insects; extracting the frozen and dried insects by using an organic solvent; carrying out GC-MS (Gas Chromatography-Mass Spectrometer) analysis on an extraction solution. The method disclosed by the invention can reduce the interference on extraction by water carried by the insects to the lowest limit, so as to increase the content of the extracted high molecular weight body wall hydrocarbon compound and improve the quality of the extracted high molecular weight body wall hydrocarbon compound, and separate components which can not be separated by using a method in the prior art; various types of high molecular weight body wall hydrocarbon compounds can be detected so as to conveniently carry out further researches on the various types of high molecular weight body wall hydrocarbon compounds.

Description

technical field [0001] The present application relates to the technical field of plant protection, in particular to an insect analysis method, and more specifically to an analysis method for hydrocarbon components in insect cuticles. Background technique [0002] Insect pheromone is a general term for compounds that play a role in chemical communication between organisms, and is the chemical component of insect communication. Insect pheromones are a class of hydrocarbons, which are chemical substances that interact between individuals of the same species, and can affect each other's behavior, habits, and even development and physiological activities. Pheromones are synthesized by glands in the body, directly excreted from the body surface, distributed on the body surface or emitted to the outside of the body. Pheromones are transmitted to other individuals by contact, air, water and other transmission media. Pheromones mainly include sex pheromones, aggregation pheromones,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张帅崔金杰王春义雒珺瑜吕丽敏
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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