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A kind of Momordica grosvenori hybrid breeding technology

A technology of hybrid breeding and Luo Han Guo, applied in plant cells, plant genetic improvement, botany equipment and methods, etc., can solve problems such as slow growth and accumulation of active ingredients

Active Publication Date: 2015-08-19
怀化兴科创生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the metabolism of polyploid plants is strong, but the growth is slow, it eventually leads to the accumulation of more organic matter, so that the active ingredients of plants, especially medicinal plants, accumulate

Method used

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  • A kind of Momordica grosvenori hybrid breeding technology
  • A kind of Momordica grosvenori hybrid breeding technology
  • A kind of Momordica grosvenori hybrid breeding technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 6

[0018] Example 1. Obtaining hexaploid Luo Han Guo female plants

[0019] With colchicine, the chromosome of the diploid Luo Han Guo female plant was doubled. In the experiment, we found that in addition to the theoretical diploid, tetraploid, there are also some abnormally doubled 5-ploid and 6-ploid (such as figure 1 , figure 2 As shown), through a large number of screenings and multiple flow cytoploidy tests, we have obtained several stable hexaploid lines.

[0020] Select female plants with vigorous growth, multiple branches, early flowering, excessive hanging, and resistance as the female parent. Bring the selected female parent material back to the laboratory, rinse with plenty of sterile water, and disinfect with 70% alcohol For 30 seconds, sterilize with 2% sodium hypochlorite for 3 minutes, rinse with sterile water 3 times, inoculate it on MS medium, and use this sterile seedling material as the induction object. The shoot tips of the above-mentioned aseptic female p...

Embodiment 2 6

[0022] Example 2. Application of hexaploid Luo Han Guo female plants in cross breeding

[0023] The hexaploid Luo Han Guo female plant z-123, etc. obtained in Example 1 were used as the hybrid female parent, and the wild-type diploid Luo Han Guo male plant material SYRC was used as the hybrid female parent. Wherein, the results of flow cytometry analysis of the male plant material of diploid Luo Han Guo as the male parent are as follows image 3 As shown, the ploidy identification results show that the material is diploid.

[0024] The hexaploid female plants obtained in Example 1 and the above-mentioned diploid Luo Han Guo male plants were planted simultaneously in spring. After flowering, it was crossed with the male parent material SYRC. The specific operation steps of the hybridization experiment are: select the well-developed male flowers on the SYRC material to be budded or slightly bloomed from 5 am to 7 am, and place them in a cool place for use. When the hexaploid fema...

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Abstract

The invention relates to a technical method for cultivating a good high-yield and high-resistance tetraploid sextuploid momordica variety by hybridizing sextuploid momordica grosvenori with diploid momordica grosvenori.

Description

technical field [0001] The invention relates to a technical method for cultivating excellent, high-yield and high-resistance tetraploid Luo Han Guo varieties by hybridizing hexaploid and diploid Luo Han Guo. Background technique [0002] Mangosteen is a rare medicinal material. Traditional Chinese medicine believes that Luo Han Guo is sweet, sour, and cool in nature. It is especially suitable for those who smoke frequently, drink alcohol, get angry easily, and those with lung function. .It is also a good health care product for friends during festivals. Luo Han Guo, as a specialty product in Guangxi, has been planted on a large scale and has high economic value. There are six varieties of Luo Han Guo: Qingpi, Lajiang, Changtan, Hongmao, Winter Melon and Chashan. Due to the advantages of high yield and strong adaptability, the planting area of ​​Qingpi fruit has reached more than 90%. [0003] Conventional hybrid breeding can combine the excellent traits scattered on dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H1/06A01H1/04
Inventor 张会新王小英
Owner 怀化兴科创生物技术有限公司
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