Strawberry subculture medium

A subculture medium and strawberry technology, which is applied in the field of plant biology, can solve the problems of unfavorable plant subculture, affecting the overall effect of the medium, and low organic matter content, so as to increase the frequency of somatic embryos and regenerated plants, The effect of improving breeding efficiency and reducing the production of secondary somatic cells

Active Publication Date: 2015-06-03
句容茂园生态农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, in terms of composition, the content of organic matter in MS basic medium is relatively low, and contains component inositol, which is not conducive to the subculture of plants. Secondly, component agar has insurmountable inherent defects in plant tissue culture, which affects The overall effect of the medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1 / 2 improved SH+2mg / L2,4-D+0.2mg / L6-BA+30g / L sucrose+4g / L phytagel, the pH value is about 5.6.

Embodiment 2

[0031] 1 / 2 improved SH+3mg / L2,4-D+0.3mg / L6-BA+30g / L sucrose+4g / L phytagel, the pH value is about 5.6.

Embodiment 3

[0033] 1 / 2 improved SH+4mg / L2,4-D+0.5mg / L6-BA+30g / L sucrose+3g / L phytagel, the pH value is about 5.7.

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PUM

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Abstract

The invention discloses a strawberry subculture medium. The strawberry subculture medium is characterized by comprising 1 / 2 improved SH+2-4mg / L2 and 4-D+0.2-0.5mg / L6-BA+30g / L saccharose+3-4g / Lphytagel, wherein the pH value is 5.6-5.8. The 2, 4-D concentration of the strawberry subculture medium is controlled, so that somatic cell formation is benefited, and the somatic cell production is reduced; moreover, the phytagel is utilized to replace agar, so that the inherent defects of conventional agar culture in plant tissues are effectively overcome, a genotype limitation problem of strawberry high-frequency somatic embryo regeneration culture is effectively solved, the somatic embryo generation frequency and the regeneration plant frequency are greatly improved, and the breeding efficiency is improved.

Description

technical field [0001] The invention relates to a medium, in particular to a strawberry subculture medium, and belongs to the technical field of plant biology. Background technique [0002] Strawberry has been promoted and planted in a large area in my country because of its outstanding economic value and ecological function. In the face of production needs and existing production conditions, strawberry needs to be further improved in terms of yield, quality and resistance. Combining biotechnology and conventional breeding methods to breed new strawberry varieties is an effective way to improve the efficiency of strawberry breeding. [0003] The traditional strawberry subculture medium is: MS basic medium + 0.4-0.5mg / L BA + 0.1-0.2IBA+ 20-30 g / L sugar + 3-4g / L agar. First of all, in terms of composition, the content of organic matter in MS basic medium is relatively low, and contains component inositol, which is not conducive to the subculture of plants. Secondly, componen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
Inventor 刘泽云
Owner 句容茂园生态农业发展有限公司
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