Light-induced Fluorescent Components Excited by Separating Microcolumns Coupled with Optical Fibers

Abstract

The invention discloses a separated micro-column coupling light guide fiber exciting light induction fluorescence component which can eliminate the background noise and improve the detection sensitivity and is mainly applicable to an instrument component for analyzing micro samples in the fields of clinical medication and life science. The separated micro-column coupling light guide fiber exciting light induction fluorescence component comprises an exciting light source (10), a separated micro-column coupling light guide fiber (30) and a fixed micro-column (40), wherein the separated micro-column coupling light guide fiber (30) comprises a light guide fiber (31) and a separated micro-column (32); the front end of the light guide fiber (31) is coupled with the exciting light source (10), and the rear end of the light guide fiber (31) extends to a detection window (41) of the fixed micro-column (40) and is coupled with the separated micro-column (32); silicon rubber (33) is filled between the separated micro-column (32) and the fixed micro-column (40), so that the liquid leakage is prevented. The separated micro-column coupling light guide fiber exciting light induction fluorescence component has the beneficial effects that the fluorescence exciting efficiency is high, and the detection sensitivity and accuracy are improved; the fluorescence component is simple in optical structure and small in size and can be combined with capillary electrophoresis and capillary liquid chromatography and other micro-column separation systems.

Description

technical field [0001] The invention relates to a detection and analysis instrument, in particular to a separation micro-column coupled optical fiber excitation light-induced fluorescence assembly mainly suitable for detection of trace samples in the fields of clinical medicine and life science. Background technique [0002] In order to adapt to the development trend of miniaturization of analytical instruments, various micro-column separation methods have emerged as the times require. High-sensitivity detection methods have always been an important content of analytical chemistry research. Therefore, improving the sensitivity of detectors has become a key issue in the practical application of micro-column separation technology. Since the inner diameter of the microcolumn is very small (generally tens to hundreds of microns), and the volume of the detection window on the column is only several to hundreds of nanoliters, the conventional ultraviolet photometric detection meth...

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Problems solved by technology

The applicant disclosed a high-efficiency capillary electrophoresis instrument in the utility model patent specification whose patent number is ZL200620034929.7 and the announcement number is CN2921831. However, the diameter of the optical fiber used is limited by the inner diameter of the capillary. Only an optical fiber with a diameter slightly smaller than the inner diameter of the capillary can be selected to make it smoothly inserted into the capillary to guide light, but too thin optical fiber is difficult to communicate with the light source. Well-coupled, even if a microscope objective lens is used to focus after the light source, the focused spot size may exceed the cross-sectional area of ​​the selected optical fiber, which will make the optical coupling incomplete, thereby affecting the fluorescence excitation efficiency and detection sensitivity

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