Lactic bacterium having an effect of ameliorating metabolic syndrome
A technology for metabolic syndrome and Lactobacillus plantarum, applied in the field of lactic acid bacteria
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example 1
[0112] Example 1: Construction of a screening system using 3T3-L1 cells derived from mouse fibroblasts
[0113] A screening system for lactic acid bacteria having anti-obesity and anti-diabetes activities using mouse fibroblast-derived 3T3-L1 cell line as an adipocyte model was established.
[0114] (1) Tested strains
[0115] Among 270 strains isolated from healthy human feces, 20 strains (strain numbers 1 to 19, 23) excellent in resistance to gastric acid or bile acid were selected and used. In addition to this, 23 strains shown in Table 1 below were used in total together with 3 strains having high immunomodulatory activity selected from a plurality of strains isolated from various fermented milks.
[0116] [Table 1]
[0117] Numbering
strain
1
MEP222801
2
MEP222802
3
MEP222803
4
MEP222804
...
example 2
[0133] Example 2: Anti-obesity and anti-diabetic activities of culture supernatants of various lactic acid bacteria skimmed milk powder
[0134] The effects of the skim milk powder culture supernatants and MRS culture supernatants of 22 strains of lactic acid bacteria numbered 1 to 21 and 23 in Table 1 on the expression level of adiponectin in 3T3-L1 cells were evaluated.
[0135] The results of a screening show that number 12 Lactobacillus amylovorus (Lactobacillus amylovorus) MEP222812 (hereinafter referred to as strain 12), number 17 Lactobacillus plantarum MEP222817 (hereinafter referred to as strain 17), number 23 Lactobacillus plantarum OLL2712 (hereinafter referred to as strain 17) The culture supernatant known as strain 23) significantly enhanced the expression level of adiponectin in 3T3-L1 cells. Furthermore, the reproducibility of these three strains was confirmed, and it was found that two strains, strain 17 and strain 23, exhibited stable effects.
[0136] In add...
example 3
[0144] Example 3: Analysis of BMDC using dendritic cells from mouse bone marrow
[0145] BMDCs were aspirated from the femurs of 7-week-old male ICRs. After the bone marrow fluid was passed through a 70 μm cell strainer, it was hemolyzed, and rabbit IgG was added to prevent non-specific adsorption. Further, add biotin-labeled anti-CD4 antibody, anti-CD8 antibody, I-A d (MHC II marker) antibody, on ice for 30 minutes. Further, streptoavidin magnetic beads and anti-220 antibody magnetic beads were added. After passing through a 40 μm cell strainer again, negative fractions were recovered using automated MACS DEPLETE. This procedure removes T cells, B cells, and antigen-presenting cells from bone marrow fluid, and isolates only immature dendritic cells.
[0146] They were cultured with RPMI (Roswell Park Memorial Institute (Roswell Park Memorial Institute))-medium 164010 ml supplemented with 10% GM-CSF (granulocyte-macrophage colony-stimulating factor), and 5 ml was added aft...
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