Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through proteolysis of eucommia seed cakes

Abstract

The invention discloses a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through the proteolysis of eucommia seed cakes. The method comprises the following steps: dissolving eucommia seed cake proteolysis powder into deionized water to prepare protein fluid; placing the protein fluid into a thermostat water bath, and conducting thermotropy treatment on protein; cooling the solution subjected to thermotropy treatment to room temperature, and regulating the pH value with 1mol/L NaOH; adding alkaline protease, dissolving and evenly mixing; placing the protein fluid into the thermostat water bath after adding enzyme, and conducting enzymolysis; increasing the temperature of the water bath after the enzymolysis is completed, heating enzymolysis fluid to modify and inactivate the protease, and finishing the enzymatic hydrolysis reaction; cooling the enzymolysis fluid to room temperature, centrifuging in a table type high-speed centrifuge, and inclining to obtain supernate; carrying out ultrafiltration on the supernate, and carrying out vacuum freeze-drying on the filtrate to obtain an ACE inhibitory peptide powder product. The method is simple to operate, and the eucommia key fruit protein is converted into functional peptide with smaller molecule mass. In addition, the ACE inhibitory peptide prepared by adopting the method has a blood pressure reduction role to a patient suffering from high blood pressure, and has no influence on people with normal blood pressure, thus having a good development prospect.

Description

technical field [0001] The invention belongs to the field of preparation of ACE inhibitory peptides, and in particular relates to a method for preparing ACE inhibitory peptides by proteolysis of Eucommia ulmoides seed dregs. Background technique [0002] Eucommia ulmoides (Eucommol / Lia Ulmoides Oliv) is a unique precious Chinese medicinal material and economic tree species in my country. It is mainly distributed in Hunan, Hubei, Shaanxi, Sichuan, Chongqing, Guizhou and other western provinces and cities. There is a large-scale Eucommia commodity production base in Wuling mountain area. There are 400,000 mu of Eucommia ulmoides in the western part of Hunan alone, with a total area of ​​more than 200,000 mu. The development and application prospects are very broad. For a long time, the use of Eucommia is limited to Eucommia bark. The fruit of Eucommia ulmoides is samara, flat, with a protein content of about 15% and 18 kinds of amino acids, among which the essential amino acids...

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Problems solved by technology

[0010] The purpose of the embodiments of the present invention is to provide a method for preparing ACE-inhibiting peptides by proteolysis of Eucommia ulmoides seed dregs, aiming a

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