General method for rapidly obtaining novel cytoplasm male sterility restoration source of rice

Abstract

The invention discloses a general method for rapidly obtaining a novel cytoplasm male sterility restoration source of rice. The method comprises the following steps: detecting the fertility of an original cytoplasm source donor according to the original cytoplasm source donor of the obtained cytoplasm male sterility material, and hybridizing the cytoplasm source donor material as a cytoplasm male sterility restoration source with the cytoplasm male sterility if the cytoplasm source donor material is normally male-fertile; and backing to the generation of the original donor material of the cytoplasm male sterility material if the cytoplasm male sterility material is male-sterile, and hybridizing the original donor material as the cytoplasm male sterility restoration source with the cytoplasm male sterility. The method allows the novel cytoplasm male sterility restoration source of rice to be rapidly obtained, is especially suitable for the like rice novel cytoplasm male sterility lines which allow good restoration sources to be not screened through a wide testcross process, and has a good repeatability and a reliable result.

Description

technical field [0001] The invention belongs to the technical field of hybrid rice breeding, and in particular relates to a general method for rapidly obtaining rice cytoplasmic male sterility restoration sources. Background technique [0002] Since the 1970s, a variety of rice cytoplasmic male sterile lines (CMS) from different sources have been bred by interspecific hybridization and nuclear replacement. At present, rice cytoplasmic male sterile lines from different sources used in production can be generally divided into wild-type (WA-CMS), Baotai-type (BT-CMS) and Honglian-type (HL- CMS) and other three major male sterile cytoplasmic types. [0003] Continuously cultivating cytoplasmic male sterile lines with new male sterile cytoplasmic sources and restorer lines with restorer gene sources is an effective technical means to avoid the potential risks of hybrid rice due to the long-term promotion of a single cytoplasmic source, and it is also an effective technical means...

AI Technical Summary

Problems solved by technology

However, in the process of cultivating new cytoplasmic male sterile lines, it is often encountered that restorer lines or restorer sources cannot be screened through extensive testing and recovery methods.

For example, the International Rice Institute uses perennial wild rice (female parent) to cross and backcross cultivated rice (hybrid and backcross male parent) to breed a new cytoplasmic male sterile line from perennial wild rice cytoplasmic male sterile source IR66707A, Jiaxing Academy of Agricultural Sciences in my country used this male sterile line as the female parent to breed Jiazao 935A, a new type of male sterile male sterile line with good recovery. So far, no recovery source has been found.

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