A general method for rapid directional breeding of new cytoplasmic male sterile restorer lines in rice

Abstract

The invention discloses a general method for rapidly and directionally cultivating a novel rice cytoplasm male sterility restoration source. The method comprises the following steps: hybridizing a novel rice cytoplasm male sterile line as a female parent and the original cytoplasm source donor material of the cytoplasm male sterile line as a male parent; carrying out composite crossing of a present rice variety as a backcross male parent with the above F1; continuously backcrossing 2-3 times, wherein an individual plant of normal fertile pollens from a backcross colony is used as female parent, and a backcross parent is used as a male parent; carrying out bagging selfing of the individual plant of normal fertile pollens in a BC3-4F1 colony to obtain BC3-4F2; and screening the line of normal fertile pollens and being similar to the backcross parent in comprehensive properties in all the BC3-4F2 lines. The above technological method has the advantages of acceleration of the novel cytoplasm male sterile rice 'three-line' matching application, good repeatability and reliable result.

Description

technical field [0001] The invention belongs to the technical field of hybrid rice breeding, and in particular relates to a general method for rapid and directional cultivation of a novel cytoplasmic male sterile restorer line of rice. Background technique [0002] Since the 1970s, a variety of rice cytoplasmic male sterile lines (CMS) from different sources have been bred by interspecific hybridization and nuclear replacement. At present, rice cytoplasmic male sterile lines from different sources used in production can be generally divided into wild-type (WA-CMS), Baotai-type (BT-CMS) and Honglian-type (HL- CMS) and other three major male sterile cytoplasmic types. [0003] Continuously cultivating cytoplasmic male sterile lines with new male sterile cytoplasmic sources and restorer lines with restorer gene sources is an effective technical means to avoid the potential risks of hybrid rice due to the long-term promotion of a single cytoplasmic source, and it is also an eff...

AI Technical Summary

Problems solved by technology

However, in the process of cultivating new cytoplasmic male sterile lines, it is often encountered that restorer lines or restorer sources cannot be screened through extensive testing and recovery methods.

For example, the International Rice Institute uses perennial wild rice (female parent) to cross and backcross cultivated rice (hybrid and backcross male parent) to breed a new cytoplasmic male sterile line from perennial wild rice cytoplasmic male sterile source IR66707A, Jiaxing Academy of Agricultural Sciences in my country used this male sterile line as the female parent to breed Jiazao 935A, a new type of male sterile line with good recovery. Restoration lines with strong advantages have not been bred

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