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Sturgeon oocyte maturation associated proteins and correlation biomaterials thereof
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A biomaterial, the technology of sturgeon roe, which is applied in the field of sturgeon oocyte maturation-related proteins and related biomaterials, can solve the problems of high cost, difficulty in obtaining, and unsatisfactory effect of oxytocic agents, etc.
Inactive Publication Date: 2015-05-27
BEIJING FISHERIES RES INST
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However, these oxytocics have their own shortcomings. For example, although the pituitary gland has a significant ripening effect, it is difficult to obtain and the cost is high; while the effects of the other two oxytocics are sometimes not ideal.
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Embodiment 1
[0062] Embodiment 1, preparation of GtHIIβα and its coding gene
[0063] 1. Construction of GtHIIβα gene expression vector
[0064] In this example, two DNA molecules encoding GtHIIβα shown in SEQ ID No.2 were artificially synthesized, and the names were GtHIIβα-A and GtHIIβα-B. The nucleotide sequence of GtHIIβα-A is SEQ ID No.1, its coding sequence is the 11-694th position of SEQ ID No.1, the 5th-10th position is the Xho I recognition site (CTCGAG), and the 698-705th position The bit is the NotI recognition site (GCGGCCGC). The nucleotide sequence of GtHIIβα-B is SEQ ID No.3, its coding sequence is the 11-694th position of SEQ ID No.3, the 5th-10th position is the Xho I recognition site (CTCGAG), and the 698-705th position The bit is the NotI recognition site (GCGGCCGC).
[0065] The PCR product of GtHIIβα-A shown in SEQ ID No.1 was digested with Xho I and Not I, ligated with the Pichia pastoris expression vector pPICZαA (Invitrogen) after the same digestion, and the liga...
Embodiment 2
[0076] Embodiment 2, promoting maturation of sturgeon oocyte
[0077] 1. Experimental materials
[0078] 1.1 Oocyte culture medium
[0079] 1.1.1 RMS (Ringer's solution modified for surgeons): NaCl6.5g, KCl0.25g, CaCl 2 0.3g, NaHCO 3 2g, dilute to 1L with water.
[0080] 1.1.2 Progesterone solution: dilute the 1mg / ml progesterone mother solution with RMS to make the final concentration of progesterone 1μg / ml, 0.5μg / ml, 0.25μg / ml respectively, to obtain 1μg / ml progesterone solution (P1 solution), 0.5μg / ml progesterone solution (P2 solution) and 0.25μg / ml progesterone solution (P3 solution).
[0081] Among them, the progesterone mother solution was prepared according to the following method: progesterone (progesterone, P) (Sigma, P0130) was dissolved in ethanol to obtain a progesterone mother solution with a progesterone concentration of 1 mg / ml.
[0082] 1.1.3 GtHIIβα solution: Dilute the 0.04 mg / ml GtHIIβα mother solution with RMS to make the final concentration of GtHIIβ...
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Abstract
The invention discloses sturgeon oocyte maturation associated proteins and correlation biomaterials thereof. The sturgeon oocyte maturation associated proteins are the proteins represented by a) or b) or c): a) the protein having an amino acid sequence represented by the 1st to the 221th in SEQ ID No. 2; b) the protein having an amino acid sequence represented by SEQ ID No. 2; c) the protein which is derived from a) and is a sturgeon oocyte maturation associated protein obtained by subjecting a) or b) to substitution and / or removing and / or adding of one or a plurality of amino acid residues. The sturgeon oocyte maturation associated proteins have biological activity of inducing sturgeon oocyte to be mature finally.
Description
technical field [0001] The invention relates to a sturgeon oocyte maturation-related protein and related biological materials. Background technique [0002] Sturgeons are a group of ancient cartilaginous scale fishes, known as living fossils, and play an important role in the history of fish evolution. At the same time, it also has important economic value, and is endangered to varying degrees around the world, all of which are listed as Appendix II species of the Convention on International Trade in Endangered Species of Animals and Flora. [0003] In order to save sturgeon resources and commercial purposes, artificial breeding of sturgeons continues to rise all over the world. However, there are many difficulties in the artificial reproduction of sturgeons: mainly because the sexual maturity of sturgeons is late, the reproductive cycle is long, and the reproductive interval is generally 2-3 years; in addition, there are asynchronous development of female gonads in both na...
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