Preparation method of high anti-oxidant milk powder addictive
An additive and high resistance technology, applied in the field of milk powder additive preparation, can solve the problems of low milk powder safety, low biological activity, complex milk powder components, etc., achieve easy control of reaction degree, high biological activity, and improve lactose intolerance Effect
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Embodiment 1
[0014] (1) Preparation of casein-lactose mixed system: According to the composition of skim milk, a casein-lactose model was constructed, and lactose was added according to the ratio of casein to lactose (1:1.6). Stir to dissolve, mix well, and adjust the pH to 6.6. Freeze-dried and crushed and stored at 4°C for later use;
[0015] (2) Preparation of casein-lactose Maillard reaction product: place the dry sample obtained in (1) in a saturated K 2 CO 3 The solution was left overnight in a humidistat. get the water activity as a w =0.44 sample. Then use microwave oven 400w to process 15min to obtain the Maillard reaction system product of casein-lactose, which has higher antioxidant activity (when the protein content is 1mg / mL, the reducing power of the system is A 700nm =0.477, DPPH clearance rate was 68%), and basically maintained its antioxidant activity during in vitro digestion.
[0016] (3) Separation: The Maillard reaction system obtained in (2) is subjected to ultr...
Embodiment 2
[0020] (1) Preparation of casein-lactose mixed system: According to the characteristic composition of milk, a casein + whey protein-lactose model was constructed, and whey protein and lactose were prepared in proportion (1:1.6) into a solution. Stir to dissolve, mix well, and adjust the pH to 8.0. Freeze-dried and crushed and stored at 4°C for later use;
[0021] (2) Preparation of casein-lactose Maillard reaction product: place the dry sample obtained in (1) in a saturated K 2 CO 3 The solution was left overnight in a humidistat. get the water activity as a w =0.44 sample. Then use a microwave oven at 400w for 15 minutes to obtain the product of the Maillard reaction system of casein-lactose, which has a high antioxidant activity. The protein content is 1mg / mL, and the reducing power of the system is A 700nm =0.378, DPPH clearance rate was 34%), and basically maintained its antioxidant activity during in vitro digestion.
[0022] (3) Separation: The Maillard reaction sy...
Embodiment 3
[0026] (1) Preparation of casein-whey protein-lactose mixed system: according to the characteristic composition of milk, a casein+whey protein-lactose model was constructed, and the casein, whey protein and lactose were proportioned (1:0.2456:1.6 ) into a solution. Stir to dissolve, mix well, and adjust the pH to 6.6. Freeze-dried and crushed and stored at 4°C for later use;
[0027] (2) Preparation of casein+whey protein-lactose Maillard reaction product: put the dry sample obtained in (1) in a saturated K 2 CO 3 The solution was left overnight in a humidistat. get the water activity as a w =0.44 sample. Then use a microwave oven at 400w for 15 minutes to obtain the product of the Maillard reaction system of casein-lactose, which has a high antioxidant activity. The protein content is 1mg / mL, and the reducing power of the system is A 700nm =0.465, DPPH clearance rate was 69%), and basically maintained its antioxidant activity during in vitro digestion.
[0028] (3) Sep...
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