Method for marking and distinguishing oil products and detecting quality of oil products
An oil and labeling technology, applied in the field of detection and analysis, can solve the problems of easy to suffer from enteritis, diarrhea, not well distinguished, difficult to accurately qualitative and quantitative, etc.
Inactive Publication Date: 2013-10-23
BGI SHENZHEN CO LTD +1
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Problems solved by technology
Although the main component of gutter oil is also triglyceride, it has more toxic substances that are pathogenic and even carcinogenic than genuine cooking oil. Long-term consumption of gutter oil will cause obvious harm to the human body, such as stunting, susceptibility to enteritis, diarrhea, Accompanied by the occurrence of pathological changes such as liver, heart, kidney enlargement and fatty liver, and even induce cancer; waste oil contains poisonous and harmful substances such as nitrate and nitrite, heavy metals, aflatoxin, wherein aflatoxin is a A strong carcinogen, its toxicity is 100 times that of the highly toxic arsenic, which shows the great harm of waste oil
[0005] The components of waste oil are relatively complex, and the specific physical and chemical indicators for its detection are still in the research stage, and it is difficult to be accurate and quantitative
The Ministry of Health has solicited 5 waste oil detection methods developed by 7 technical institutions, but after expert demonstration, it was found that these methods are not very specific
The so-called low specificity means that these methods do not distinguish well which is gutter oil and which is qualified edible oil
Method used
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Embodiment 1
[0074] Example 1 waste oil detection
[0075] (1) extraction of nucleic acid in waste oil:
[0076]Add 1.5ml simulated waste oil and 300ulTE buffer into a 2ml centrifuge tube, shake and mix for 5min, centrifuge at 13000rpm for 5min, discard the upper oil phase, then add 1.5ml waste oil, repeat the operation 30 times, and the collected water phase is used for nucleic acid extract. Add 600ul lysate to 300ul water phase, vortex to mix, add 900ul chloroform / isoamyl alcohol (24:1), shake vigorously for 5min, centrifuge at 13000rpm for 5min, take the upper aqueous phase and add 1 times the volume of pre-cooled isopropanol, - Place at 20°C for 1h. Centrifuge at 13000rpm for 30min, discard the supernatant, add 1ml of pre-cooled 75% ethanol, centrifuge at 13000rpm for 5min, remove the supernatant, dry at room temperature, add 20ulTE to dissolve.
[0077] (2) Target region amplification
[0078] Primers:
[0079] CYTB primer
[0080] Forward primer: 5'-TCTTGCAAATCCTAACAGGC-3'
[...
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The invention relates to the field of detection and analysis, and in particular relates to a method for marking and distinguishing oil products and detecting the quality of the oil products by using nucleic acid sequence information in the oil products. According to the method, a marker is obtained by measuring at least part of sequence information of at least part of nucleic acid molecules and fragments and marking the oil products on the basis of the sequence information. The oil products are marked and distinguished and the quality of the oil products is detected on the basis of the marker. The method is high in sensitivity and strong in practicability and can realize a high flux.
Description
technical field [0001] The invention relates to the field of detection and analysis, in particular to a method for classifying and quality testing oil products, and more specifically to a method for marking, distinguishing and quality testing oil products using genetic material in the oil products. Background technique [0002] The classification standards of oil products, such as edible oil, now mostly rely on chemical analysis indicators such as acid value, peroxide value, and solvent residue. However, these indicators are still insufficient to distinguish between different types of oil products. For example, waste oil, which is currently hotly discussed in the society, cannot be well distinguished under the existing classification standard system. [0003] Waste oil (also known as swill oil or swill oil) is a general term for various inferior oils and fats in people's life. Waste oil in a narrow sense refers to cooking residues, leftovers, tableware washing water, etc. ...
Claims
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IPC IPC(8): C12Q1/68C12N15/11C12N15/10
Inventor 李计广章文蔚龚梅花韩鸿雁
Owner BGI SHENZHEN CO LTD