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Integrated micro-optics structural array device

A technology of micro optics and light concentrating structure, applied in the field of biotechnology detection, can solve the problems of cumbersome operation, waste, and low efficiency of fluorescence collection, improve accuracy and stability, simplify time and workload, and realize automatic operation Effect

Inactive Publication Date: 2013-10-23
WUXI G S PRECISION TOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. The operation is cumbersome, such as sticking the chip fence and cleaning the glass slides, all of which are manual operations, which is not conducive to the accuracy and stability of the reaction
[0005] 2. The detection is not flexible, and the entire slide must be used each time. If the number of samples is relatively small, it will cause waste
[0006] 3. The organic molecular film coated on the glass surface is easy to fall off, resulting in unstable experimental results
[0007] 4. The planar structure of the glass slide leads to low fluorescence collection efficiency, the glass surface is not easy to do chemical treatment, and the background signal of the glass is high, which makes it unfavorable for high-sensitivity detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Such as figure 1 Schematic diagram of the structure and figure 2 As shown in the longitudinal sectional view of FIG. 1 , in this embodiment, the integrated micro-optical structure array device is composed of a slat 100 and a plate frame 200 integrated with the micro-optical structure array. The structure of the strip 100 is the same as the enzyme label strips used in ELISA (enzyme-linked immunosorbent assay) in the prior art, which can be of common specifications such as 8 holes, 12 holes, 16 holes, etc., and can be disassembled into any holes The number is placed on the frame. The single hole structure 110 of the strip 100 can be in the shape of a circle or a square, etc., and the hole spacing is the same as that of a standard microwell plate. The inner surface 112 of each pore structure 110 is surface-treated and has active groups that can bind to biomolecules, such as aldehyde groups or epoxy groups that can bind to protein samples, etc., and a micro-optical struc...

Embodiment 2

[0055] Such as image 3 and Figure 5 As shown, in this embodiment, the integrated micro-optical structure array device is composed of a solid substrate 300 and a frame 400 with holes on which the micro-optical structure array is integrated on the surface.

[0056] The size of the solid substrate 300 can be 1 inch*3 inches, 0.72 inches*3 inches, 2 inches*6 inches, etc., and its material can be glass or polymer material. The micro-optical structure array 310 is integrated on the surface of the solid substrate, and the two are integrally processed and formed. Such as Figure 4 As shown, the density and position of the micro-optical structure array 310 can be determined according to requirements. The perforated frame 400 can be standard 16-hole, 24-hole, 48-hole, 72-hole or 96-hole and other commonly used specifications. The hole 410 can be in the shape of a circle or a square, and the hole is a through hole and has no bottom.

[0057] The solid base 300 and the perforated fr...

Embodiment 3

[0061] Such as image 3 and Figure 9 As shown, in this embodiment, the integrated micro-optical structure array device is composed of a solid substrate 300 and a chip fence 600 on which the micro-optical structure array is integrated on the surface.

[0062] The size of the solid substrate 300 can be 1 inch*3 inches, 0.72 inches*3 inches, 2 inches*6 inches, etc., and its material can be glass or polymer material. The micro-optical structure array 310 is integrated on the surface of the solid substrate, and the two are integrally processed and formed. The chip fence 600 is made of rubber material, which is soft and easy to stick on the surface of the solid substrate 300. Its function is mainly to isolate samples and avoid cross-contamination, and is suitable for occasions where there are few samples to be tested.

[0063] The solid substrate 300 of the integrated micro-optical structure array 310 is firstly surface-treated to have active groups on the surface, and then sampl...

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Abstract

An integrated micro-optical structure array device comprises a solid substrate (300) integrated with micro-optical structure arrays (310) and a frame (400). Multiple micro-optical structure arrays (310) exist, and each micro-optical structure array (310) is arranged in the form of an array on a plane. The frame (400) is combined on the solid substrate (300) and separates the micro-optical structure arrays (310). A micro-optical structure (111) is a light diverting structure or is formed by integrating a light diverting structure and a light condensing structure. The light diverting structure is a circular table structure (111c), a square column structure, a hexagonal column structure, or an octahedral column structure, and the light condensing structure is a convex lens (111e) or a Fresnel lens (111d). The size of the device is the same as that of a standard microplate. One-to-one correspondence exists between the positions of micro-optical structure arrays (310) and holes on the standard microplate. The micro-optical structure improves the signal collecting efficiency, and a standard microplate structure thereof is compatible with an existing device, and can implement automation in the whole detection process.

Description

technical field [0001] The invention relates to the field of biotechnology detection, and constructs a micro-analysis system by immobilizing target molecules on the surface of a micro-optical structure, and in particular relates to an integrated micro-optic structure array device. Background technique [0002] In the prior art, microarray technology is a plane carrier on which many units are neatly arranged in rows and columns, and genes or protein molecules are regularly and specifically adsorbed in each unit. An analysis device is called a microarray, and it should meet three requirements: ① it is planar, ② it is regular, and ③ it is on a microscopic scale. The microscopic scale of the microarray enables the reaction to occur rapidly in terms of reaction kinetics, thereby realizing the rapid detection of a large number of indicators. [0003] At present, microarray chips usually use standard glass slides (1 inch * 3 inches), however, microarray chips using glass slides ha...

Claims

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Application Information

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IPC IPC(8): G01N21/01
CPCG01N21/01G01N21/0303G01N2021/0346G02B3/0037G02B5/0215G02B19/0028G02B19/0047
Inventor 王振宇董凌志梁银针马贵兰戴良
Owner WUXI G S PRECISION TOOL