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Test strip for testing sulfanilamide drug residues and application method

A technology of sulfonamide drugs and immunochromatographic test paper, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of uneven composition and structure of chromatographic test strips, batch inhomogeneity, solution chromatography and immunological reaction differences, etc. question

Inactive Publication Date: 2013-10-23
QINGDAO BOITE BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The HPLC method has high detection sensitivity and specificity, but its operation is cumbersome
Physical and chemical detection methods are complicated in procedure and high in analysis costs, and are not suitable for the detection of a large number of samples. Microbiological methods are time-consuming and have low sensitivity
[0005] At present, colloidal gold immunochromatography is the most commonly used method in the analysis of sulfonamide veterinary drug residues. However, due to the limitation of the optical properties of the detection marker colloidal gold molecules, this method can only realize the qualitative detection of sulfa drugs. It is difficult to achieve a qualitative measurement of its residues, and in the prior art, due to the wide variety of detection samples, the pH value, ion concentration and protein content are different, which will inevitably have different effects on the release of the binding pad indicator substance. In addition, the nitrocellulose Inhomogeneity in the composition and structure of chromatography test strips such as membranes will lead to differences in the chromatography and immune response of the solution. Different batches of the same chromatography test strip may have greater heterogeneity, that is, the difference between batches CV

Method used

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  • Test strip for testing sulfanilamide drug residues and application method
  • Test strip for testing sulfanilamide drug residues and application method
  • Test strip for testing sulfanilamide drug residues and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Taking sulfamethazine SMZ as an example to verify the specificity of the immunochromatographic test strip prepared by the present invention

[0035] 1. The SMZ-specific monoclonal antibody and rabbit anti-mouse IgG secondary antibody used in this experiment are commercial reagents purchased from SIGMA.

[0036] 2. Handling of samples to be tested

[0037] The processing method of pork, chicken and crucian carp: Weigh 2g of homogeneous sample into a glass bottle, add 4ml of methanol, mix vigorously for 2min, and filter with filter paper.

[0038] Milk treatment method: Measure 1ml milk, add 2ml PBST and mix well, then add 60μl 1mol / Lde zinc sulfate solution, mix for 1min, then add 60μl 0.36mol / L potassium ferrocyanide solution, shake for 1min, filter and use.

[0039] 3. Choose from 15 sulfa drugs (STZ, SIZ, SME, SMPD, SP, SMT, SCP, SD, SMR, SM, SMX, SDZ, SDMX, SIX and SQ) other than SMZ and other common veterinary drugs: chloramphenicol, Specific detect...

Embodiment 2

[0042] Embodiment 2: adopt the chromatographic test strip of the present invention to detect the lower limit of the detected concentration of 16 kinds of sulfonamides, and the results show that the chromatographic test strip with the quantum dot label and the microchannel result of the present invention is adopted It has high sensitivity to these sulfa drugs and can be widely used in the determination of multiple drug residues.

[0043] Table 2: The lower limit of concentration of 16 kinds of sulfonamides detected by test strips of the present invention

[0044] Sulfa drug name Concentration lower limit μg / L STZ 1.5 SIZ 2 SMEs 3 SMPD 3 SP 3 SMT 5 SCP 5 SD 5.5 SMR 6 SM 8 SMX 8 SDZ 10 SMZ 20 SDMX 50 SIX 60 SQ 90

Embodiment 3

[0045] Embodiment 3: CV detection of difference between batches of test strips of the present invention

[0046] Select the colloidal gold immunochromatography test strip that buys on the market as contrast, analyze the batch-to-batch difference of the chromatography test strip that has microchannel according to the present invention, and analysis result is as shown in table 3, and analysis result shows that adopts The batch-to-batch difference of the microfluidic chromatography test strip is significantly reduced, and the analysis results obtained by using it for detection are more stable and reliable.

[0047] Table 3: Results of CV analysis of the differences between batches of test strips

[0048]

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Abstract

The invention provides an immunochromatographic test strip for testing sulfanilamide drug residues. The test strip is characterized in that a sample applying zone, a reaction capturing zone and a remaining liquid absorbing zone are arranged on the substrate of the test strip. Specific monoclonal antibodies of sulfanilamide drugs and labeled with quantum dots are fixed in the sample applying zone. Test strips containing specific monoclonal antibodies of sulfanilamide drugs and quality control strips containing rabbit anti rat IgG antibodies are arranged in the reaction capturing zone. The sample applying zone, the reaction capturing zone and the remaining liquid absorbing zone are located on downward-recessed micro flow channels of the substrate of the test strip.

Description

technical field [0001] The invention relates to a test strip for detecting residues of sulfonamide drugs and a using method thereof. [0002] Background technique [0003] Since the first synthesis of the red azo dye Prontosil containing sulfonamide groups in 1932, its development has experienced more than 80 years of history. It has the advantages of consuming food and being able to produce in large quantities in China, so far it is considered one of the important drugs for anti-infection treatment of livestock and poultry. As feed additives, after the breeding industry uses animal medicines marked with withdrawal periods and feed additives containing medicines, if the appropriate withdrawal period is not observed, the muscle tissue, eggs and milk will be contaminated by residual sulfonamides . Short-term high-dose or long-term low-dose administration can easily cause the accumulation of sulfa drugs in various tissues of animals. When feed or drinking water is contamina...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 凌红丽王睿智蒋贻海
Owner QINGDAO BOITE BIOPHARM