Trichoderma harzianum Sch234 bacterial strain as well as preparation method and application thereof

A technology of bacterial strains and bacterial agents, applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as difficult to prevent, difficult to reach, and inconvenient movement, and achieve labor saving, simple application methods, The effect of simple production technology
CN103374527AInactive Publication Date: 2013-10-30HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2013-10-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses trichoderma harzianum Sch234 bactericide as well as a preparation method and an application thereof. The preparation method of the trichoderma harzianum Sch234 bactericide comprises the following steps of: (1) firstly activating a trichoderma harzianum bacterial strain Sch234 on a PDA flat plate, then inoculating the trichoderma harzianum bacterial strain Sch234 on a PDA inclined plane to be cultured, adding deionized water for sterilization, preparing spore suspension, adding tween-20 until final concentration of the tween-20 in the spore suspension is 0.025%, and adjusting the concentration of the spore suspension to be 1.5*10<6> / ml; (2) mixing dry substrate oatmeal with rice husk uniformly, adding water, and carrying out high-pressure steam sterilizing; and (3) inoculating the prepared spore suspension with substrate in ratio of 10ml per bag, cultivating, and drying in the air and grinding, thus the trichoderma harzianum Sch234 bactericide is obtained. The trichoderma harzianum bacterial strain Sch234 has a strong parasitic effect on rice sheath blight disease and hypha and sclerotium of sclerotinia sclerotiorum, control effect of antibiological inoculant to rice sheath blight disease under field cultivation condition is 83%, an application method in soil treatment is simple, labour force is saved, and the trichoderma harzianum bacterial strain Sch234 can be used for producing biopesticide and is safe to people and livestock.
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Description

technical field

[0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a bacterial strain Trichoderma certzii Sch234 strain used for the biological control of rice sheath blight and rape sclerotinia, and also relates to the preparation of a biocontrol bacteria Trichoderma harzianum Sch234 bacterial agent The method also relates to the application of a Trichoderma harzianum Sch234 strain in the preparation of medicines for treating or preventing rice sheath blight and rape sclerotinia. Background technique

[0002] Plants will be infected with various diseases during the growth process, which will reduce the yield and quality to varying degrees. Most of the pathogenic bacteria that cause plant diseases are fungi, which cause more than 85% of plant diseases, such as rice blast, sheath blight, wheat rust, powdery mildew, rape sclerotinia and other important crops. The most serious diseases are caused by Caused ...

Claims

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