Method for determination of caffeic acid content of main flue gas of cigarette by high performance liquid chromatography-tandem mass spectrometry
A high-performance liquid chromatography and cigarette mainstream smoke technology, applied in the field of tobacco and chemistry, can solve problems such as low caffeic acid content, matrix interference, complex smoke components, etc., and achieve high sensitivity and fast method effects
Active Publication Date: 2013-11-20
CHINA TOBACCO JIANGSU INDAL
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Problems solved by technology
[0006] During the combustion process of cigarettes, various physical and chemical reactions such as distillation, dry distillation, pyrolysis, synthesis, and condensation occur, and a large number of nascent compounds are formed. What method is used to measure the content of caffeic acid in mainstream smoke of cigarettes? Relevant literature There are few reports. Up to now, no patents or literature reports on the determination of caffeic acid content in mainstream cigarette smoke by high performance liquid chromatography-tandem mass spectrometry have been found at home and abroad
Moreover, the smoke composition is very complex, the matrix interference is serious, and the content of caffeic acid in the smoke is low. Whether the established detection method for phenolic compounds in the smoke can be applied to the determination of caffeic acid in the smoke is not yet known.
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Embodiment 1
[0036] The method for measuring caffeic acid content in cigarette mainstream smoke by high performance liquid chromatography-tandem mass spectrometry comprises the following steps:
[0037] Sample collection: Balance the cigarettes at (22±1)°C and relative humidity (60±3)% for 48 hours, and then smoke the cigarettes according to the conditions specified in the tobacco industry YC / T29-1996 standard, using glass fiber filters Mainstream smoke was captured, and 20 cigarettes were smoked in each group.
[0038] Filter extraction: Put the filter with the particulate matter of 20 cigarettes in a 150mL conical flask, add 40mL of methanol solution, and place it on a speed-adjustable shaker to vibrate for 30 minutes.
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The invention discloses a method for determination of caffeic acid content of main flue gas of a cigarette by high performance liquid chromatography-tandem mass spectrometry. The method comprises the following steps of catching caffeic acid in main flue gas of a cigarette by a filter sheet, carrying out extraction of the filter sheet by methanol to obtain extract, detecting the extract by high performance liquid chromatography-tandem mass spectrograph, and carrying out quantitative analysis of caffeic acid content by an external standard method. The method has the advantages of simple pre-treatment processes, good separation effects, accurate qualitative analysis, short sample analysis time, and high detection sensitivity.
Description
technical field [0001] The invention relates to the technical fields of tobacco and chemistry, in particular to a method for measuring caffeic acid content in cigarette mainstream smoke by high performance liquid chromatography-tandem mass spectrometry. Background technique [0002] Caffeic acid is a compound that widely exists in natural plants and has significant biological activity. It has a wide range of antibacterial effects. In vitro tests have shown that it has antiviral activity, as well as hemostatic and detoxification effects. Therefore, the effect of this compound on the human body Health effects have been widely concerned. However, at the molecular level, the compound can cause breaks in the double-strand structure of DNA in vitro experiments. At the cellular level, the compound was found to cause chromosomal aberrations and sister chromosome exchange, according to the International Agency for Research on Cancer of the World Health Organization (IARC) classified...
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IPC IPC(8): G01N30/89
Inventor 庄亚东朱怀远秦艳华尤晓娟张媛盛金刘献军曹毅朱莹韩开冬沈晓晨熊晓敏张华
Owner CHINA TOBACCO JIANGSU INDAL