A kind of preparation technology of vancomycin crystal

Abstract

The invention provides a preparation technology for vancomycin crystals. The preparation technology comprises the following steps of after microfiltration of vancomycin fermentation liquor, adsorbing and eluting through cation exchange resin, adding urea into the obtained high concentration eluant of the vancomycin, stirring and dissolving, then filtering and removing suspended particles, standing and crystallizing, and filtering so as to obtain the crude vancomycin crystals. The preparation technology is simple to operate, the obtained crystals are grey white, the grain size of the crystals is large (the average grain diameter detected by a microscope is about 32-47mu m), and the obtained crystals are easy to filter; the crystallization yield is more than 85%, the liquid phase purity of the vancomycin in a crystal dissolving solution is more than 85%, and the color of the solution is light; and the generated urea can be taken as a microorganism nitrogen source, thus avoiding contaminating the environment.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of antibiotics, and in particular relates to a preparation process of vancomycin crystals. Background technique [0002] Vancomycin is a glycopeptide antibiotic isolated from the fermentation broth of a strain of Amycolatopsis orientalis by Micormick et al. in 1956. It was developed by Eli Lilly of the United States and approved by the FDA in 1958. In the 1980s, with the extensive use of β-lactam antibiotics, infections caused by methicillin-resistant Staphylococcus aureus (MRSA) became prevalent. Vancomycin is an important drug used clinically to treat severe infectious diseases caused by MRSA, and it has attracted more and more attention. The chemical structure of vancomycin is shown in the following formula: [0003] [0004] U.S. Patent Publication No. 4440753 discloses the use of solvents such as isopropanol, ethanol or acetone to precipitate vancomycin, but this meth...

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Problems solved by technology

[0004] U.S. Patent Publication No. 4440753 discloses the use of solvents such as isopropanol, ethanol or acetone to precipitate vancomycin, but this method uses a large amount of solvent, resulting in too dark a precipitate color, and the solvent has a large environmental pollution; the U.S. Patent Publication No. 5235037 The Chinese patent with the patent and publication number 101260134A adopts the salting-out method of adding alkali metal salt and ammonium salt to vancomycin solution to precipitate vancomycin respectively. Deep, the sediment particle size is too fine, the sediment is difficult to filter

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