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Preparation and culture method of Cordyceps culture medium, and application thereof

A technology for Cordyceps sinensis and culture medium is applied in the field of preparation of Cordyceps sinensis culture medium, which can solve the problems of slow growth of mycelium and long cultivation time, and achieve the effects of good growth and short cultivation time.

Inactive Publication Date: 2015-06-24
东方中科生命科学有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, scientific research workers have published some related patents and papers, disclosing some solid medium and cultivation methods of Cordyceps sinensis, but there are generally problems such as long cultivation time and slow mycelium growth.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1. Applied to the mother species preparation in Cordyceps sinensis fermentation factory

[0025] Harvest fresh Cordyceps sinensis (the Latin scientific name is Cordyceps sinensis, also written as Ophiocordyceps sinensis) from the place of origin of Cordyceps sinensis, insert the compact tissue of Cordyceps sinensis into the solid medium of the present invention, put it into a light incubator, and according to the natural growth conditions of Cordyceps sinensis According to the actual light conditions under the environment, it is set as continuous light for 12 hours and then continuous darkness for 12 hours, and so on, and the culture temperature is 10-20°C. After 30 days of cultivation, the diameter of the Cordyceps fungus can reach 4-6 cm.

Embodiment 2

[0026] Example 2. Applied to the preservation of strains in the fermentation factory of Cordyceps sinensis

[0027] In the Cordyceps fermentation factory, the Cordyceps solid medium of the present invention can be applied to the preservation of the Cordyceps strains, and the Cordyceps strains are transferred to the solid medium of the present invention with an inoculation needle, and cultivated in a completely dark environment as required , when cultured for 40 days, the diameter of the Cordyceps sinensis strain can reach 3-5 cm, and the medium still has strong water retention, which can be kept or transferred.

Embodiment 3

[0028] Example 3. The strain rejuvenation applied in the fermentation factory of Cordyceps sinensis

[0029] In the Cordyceps sinensis fermentation factory, strain degradation is a serious problem, and strain rejuvenation is an effective means to overcome this problem. The Cordyceps sinensis solid culture medium and the cultivation method described in the present invention can be used for the rejuvenation of strains, and the cultivation method is the same as in Example 1. After 3-5 generations are transferred, the Cordyceps sinensis can be grown very well and the rejuvenation effect is remarkable.

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PUM

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Abstract

The invention discloses a solid medium for Cordyceps sinensis and a cultivation method thereof, which solves the problems of slow growth and long cycle of solid cultivation of Cordyceps sinensis. The solid medium is mainly composed of corn flour, potato, tryptone, yeast extract powder, silkworm chrysalis powder, sodium chloride, magnesium sulfate, potassium dihydrogen phosphate, vitamins, agar powder, and water. The Cordyceps sinensis solid culture medium and the culture method thereof provided by the invention can promote the mycelia growth of the Cordyceps sinensis solid culture, shorten the culture time, and can be applied to the preservation of the Cordyceps sinensis strains in the fermentation factory, the preparation of the mother species, the activation of the strains, and the rejuvenation of the strains and other steps.

Description

technical field [0001] The invention belongs to a preparation method, cultivation method and application of a Cordyceps sinensis culture medium. Background technique [0002] Cordyceps sinensis is used in traditional Chinese medicine to invigorate the lungs and kidneys, reduce phlegm and relieve cough, and it is recorded in "Materia Medica Congxin" and "Pharmacopoeia of the People's Republic of China". It is reported that Cordyceps sinensis has anti-tumor, anti-fatigue, anti-oxidation, improving immunity, enhancing kidney function and other effects, and has become a hot spot in the current research and development of traditional Chinese medicine. [0003] The solid culture of Cordyceps sinensis is the basis of the artificial cultivation and fermentation of Cordyceps sinensis, which can be used in the steps of separation and preservation of the strains of Cordyceps sinensis, and can also be used in the steps of preparation of mother seeds and strain rejuvenation in the indust...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G1/00
Inventor 李文新陈春森廖金永
Owner 东方中科生命科学有限责任公司