Preparation method of total lactone of inula helenium L
A technology of inulin and total lactones, applied in the field of biomedicine, can solve the problems of high cost, unstable process, inability to ensure extraction of inulin sesquiterpene lactones, etc., and achieve the effects of cost saving and efficiency improvement
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Embodiment 1
[0027] This embodiment is implemented under the following conditions of implementation and technical requirements:
[0028] 1) Grind 650g of dried vetiver roots into coarse powder, add 5200mL of 70% ethanol, and soak for 6h.
[0029] 2) Heat the medicinal material and solvent in step 1) to reflux for 3 hours, recover the solvent, and obtain 156.65 g of inulin extract, which is tested by UPLC and has a purity (calculated based on the sum of the contents of inulin and inulin) was 8.17%.
[0030] 3) Perform silica gel column chromatography on the alcohol extract in step 2), using silica gel with a particle size of 100-200 mesh, the sample amount is sample: silica gel 1:2 (w / w), and the diameter-to-height ratio is 1:2 , Petroleum ether: acetone (100:2, v / v) 9100mL elution, collect the eluate.
[0031] 4) The eluate in step 3), the solvent was recovered, and dried under reduced pressure to constant weight to obtain 11.13 g of refined total lactones. After UPLC detection, its pur...
Embodiment 2
[0033] This embodiment is implemented under the following conditions of implementation and technical requirements:
[0034] 1) Grind 653g of dried vetiver roots into coarse powder, add 7836mL of 85% ethanol, and soak for 15h.
[0035] 2) Heat the medicinal material and solvent in step 1) to reflux for 2 hours, and recover the solvent to obtain 162.34 g of inulin extract, whose purity (calculated based on the sum of the contents of inulin and inulin) was detected by UPLC ) was 7.16%.
[0036] 3) The alcohol extract in step 2) is subjected to silica gel column chromatography, using silica gel with a particle size of 100-200 mesh, the sample amount is sample: silica gel 1:2.5 (w / w), and the diameter-to-height ratio is 1:3.5 , petroleum ether: acetone (100:3, v / v) 7836mL eluent, collect the eluate.
[0037] 4) The eluate in step 3), the solvent was recovered, and dried under reduced pressure to constant weight to obtain 11.18 g of refined total lactones. After UPLC detection, i...
Embodiment 3
[0039] This embodiment is implemented under the following conditions of implementation and technical requirements:
[0040] 1) Grind 650.8g of dried vetiver roots into coarse powder, add 7809.6mL of 95% ethanol, and soak for 24 hours.
[0041] 2) Heat the medicinal material and solvent in step 1) to reflux for 1 hour, and recover the solvent to obtain 158.86 g of inulin extract, whose purity (calculated based on the sum of the contents of inulin and inulin) was obtained by UPLC ) is 7.94%.
[0042] 3) The alcohol extract in step 2) is subjected to silica gel column chromatography, using silica gel with a particle size of 100-200 mesh, the sample amount is sample: silica gel 1:3 (w / w), and the diameter-to-height ratio is 1:5 , petroleum ether: acetone (100:4, v / v), eluted in 6508mL, and collected the eluate.
[0043] 4) The eluate in step 3), the solvent was recovered, and dried under reduced pressure to constant weight to obtain 11.16 g of the refined product of total lacton...
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