Recombinant newcastle disease virus rAI4-gB vaccine strain expressing infectious laryngotracheitis virus gB protein, and construction method for the vaccine strain

Abstract

The invention relates to a recombinant newcastle disease virus (NDV) rAI4-gB vaccine strain expressing an infectious laryngotracheitis virus gB protein, and a construction method for the vaccine strain. The accession number of the newcastle disease virus rAI4-gB vaccine strain is CGMCC No: 8053. The construction method includes inserting a sequence showed as SEQ ID NO.7 into an AI4 genome full-length transcripted vector pNDV / AI4, by utilizing an established reverse genetics operation platform of gene VII-type NDV attenuated AI4 strains, so as to obtain recombinant newcastle disease virus genome full-length cDNA cloned pNDV / AI4-gB comprising the infectious laryngotracheitis virus gB gene. Recombinant virus rAI4-gB obtained by transfection has high reproducing titer in a chick embryo, and is capable of stable expression of the gB protein even after continuous passage and is suitable for large-scale production of the vaccine. The recombinant virus rAI4-gB can be used for manufacturing the vaccine.

Description

technical field [0001] The invention relates to the application of reverse genetic technology to rescue a recombinant vaccine strain rAI4-gB expressing the gB protein of infectious laryngotracheitis virus with Newcastle disease virus as a carrier, and is used for vaccine development. Background technique [0002] Reverse genetics manipulation technique (Reverse genetics manipulation technique) refers to the reverse transcription of RNA virus genomic RNA into cDNA, and the artificial construction of new infectious viruses in vitro by using methods such as gene mutation, gene insertion, gene knockout, and gene replacement. , to study the genome structure and function of the virus and the interaction between the virus host [Conzelmann KK. Also often referred to as "virus rescue". With the establishment of reverse genetic technology for Newcastle disease virus, the "rescued" RNA virus is derived from cDNA clones. Therefore, through the artificially added DNA link in the interme...

AI Technical Summary

Problems solved by technology

However, inactivated vaccines cannot provide sufficient protection, and oil-emulsion adjuvants can cause local adverse reactions, resulting in decreased meat quality and animal discomfort. In addition, there are many types of vaccines that need to be vaccinated during the growth of poultry, and some vaccines need to be administered twice or even multiple times. The immunization of poultry causes stress to the poultry and affects the growth in different degrees; at the same time, the vaccination often interferes with the serological monitoring of the disease. have serious consequences

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