A kind of lignin enzyme and production method

Abstract

The invention discloses a production method for industrially producing ligninase by adopting a bacterial liquid deep submerged fermentation technology. The production method is characterized by comprising the following steps of: culturing a strain, performing bottle shaking fermentation and preparing an enzyme solution, wherein the strain is Bacillus altitudinis with a laboratory serial number of KERR7989 and a preservation number of CGMCC NO:6225, the ligninase is an alkaline enzyme, does not generate cellulase, does not have activity to crystalline cellulose and carboxymethyl cellulose, the pH range for enzyme action of the ligninase is 4.5-11.0, and the operation temperature range is 20-75 DEG C. The ligninase disclosed by the invention is high in activity, the concentration of a standard ligninase solution is more than or equal to 500u / ml, the unit production cost is low, the fermentation cycle is short, the degree of industrialization is high, and the technical process is simple and easy to control, so that ligninase is suitable for application in the papermaking industry, can reduce the papermaking production cost and environmental pollution, is helpful for improving the product quality of paper at the same time, and can be used in the fields of sewage engineering, biological enzyme preparation degradation of toxins and the like.

Description

technical field [0001] The invention relates to the industrialized production of a biological enzyme preparation, in particular to a ligninase and a production method. Background technique [0002] Ligninase is a general term for a class of enzymes with the ability to catalyze the oxidation and degradation of lignin. This type of enzyme catalyzes redox reactions. The system is called oxidoreductase, and the common name is dehydrogenase. It includes four components: It is called the diphenol oxidoreductase, which acts on the oxidation reaction of o-quinol and p-quinol, aminophenol and diamine; The oxidation reaction of manganese peroxidase, which is called divalent manganese hydrogen peroxide oxidoreductase, catalyzes the oxidation of divalent manganese into trivalent manganese, which is the oxidative degradation of lignin; diarylpropane peroxidase is also It is called ligninase I, that is, the system name is diarylpropane: oxygen, hydrogen peroxide oxidoreductase, which cat...

AI Technical Summary

Problems solved by technology

However, the fermentation is not uniform, the growth of bacteria, the absorption of nutrients and the secretion of metabolites are uneven everywhere, which makes the detection and control of fermentation parameters (pH, temperature, culture conditions, etc.) difficult, and it is difficult to realize industrialization