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Active glycoprotein extracted from purslane and preparation method thereof

A technology of purslane and glycoprotein, applied in the field of active glycoprotein and its preparation, can solve the problem of less active glycoprotein and achieve the effect of high yield and high purity

Inactive Publication Date: 2015-04-15
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are many studies on purslane polysaccharides, but few studies and reports on active glycoproteins and their extraction methods. In particular, how to ensure the activity of glycoproteins while improving yield and purity has always been an issue. Weaknesses in prior art

Method used

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  • Active glycoprotein extracted from purslane and preparation method thereof
  • Active glycoprotein extracted from purslane and preparation method thereof
  • Active glycoprotein extracted from purslane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Steps to extract active glycoprotein:

[0028] (1) Take 1g of fresh Portulaca oleracea, wash it, add 2ml of NaCl solution, grind to a slurry, add 8ml of NaCl solution, and stir evenly to obtain a slurry;

[0029] (2) Stir the above-mentioned grinding solution at 200 rpm for 4 hours, and then centrifuge at 3000 rpm for 20 minutes to obtain the supernatant and precipitate;

[0030] (3) Take the above precipitate, weigh the weight of the precipitate to be 0.8g, add 4ml of NaCl solution, and centrifuge at 3000rpm for 20min to obtain the supernatant and precipitate;

[0031] (4) Repeat step (3) to obtain the supernatant, and combine the supernatants to obtain the extract;

[0032] (5) Use an ultrafilter to concentrate and desalinate the above-mentioned extract. When the volume of the extract is reduced to one-fifth of the original volume, it becomes a concentrated liquid, wherein the molecular weight cut-off of the ultrafilter is 5000 Daltons;

[0033] (6) Add anhydrous ethanol at ...

Embodiment 2

[0040] 1. Steps to extract active glycoprotein:

[0041] (1) Take 2g of fresh purslane, wash it, add 4ml of NaCl solution, grind to a slurry, add 16ml of NaCl solution, stir evenly to obtain a grinding solution, the mass percentage of the NaCl solution is 4.5%;

[0042] (2) Stir the above-mentioned grinding solution at 200 rpm for 4 hours, and then centrifuge at 3000 rpm for 20 minutes to obtain the supernatant and precipitate;

[0043] (3) Take the above precipitate and weigh the weight of the precipitate as 1.6 g, add 8 ml of the NaCl solution described in step (1), and centrifuge for 20 min at 3000 rpm to obtain the supernatant and the precipitate;

[0044] (4) Repeat step (3) 3 times to obtain the supernatant, and combine the supernatants to obtain the extract;

[0045] (5) Use an ultrafilter to concentrate and desalinate the above-mentioned extract. When the volume of the extract is reduced to one-fifth of the original volume, it becomes a concentrated liquid, wherein the molecular...

Embodiment 3

[0053] 1. Extraction steps of active glycoprotein:

[0054] (1) Take 1g of fresh Portulaca oleracea, wash it, add 4ml of NaCl solution, grind to a slurry, add 8ml of NaCl solution, stir evenly to obtain a grinding solution, the mass percentage of the NaCl solution is 4.5% ;

[0055] (2) Stir the above-mentioned grinding solution at 200 rpm for 4 hours, and then centrifuge at 3000 rpm for 20 minutes to obtain the supernatant and precipitate;

[0056] (3) Take the above precipitate and weigh the precipitate to weigh 0.7g, add 3.5ml of the NaCl solution described in step (1), repeat step (2) to obtain the supernatant and precipitate;

[0057] (4) Repeat step (3) twice to obtain the supernatant, and combine the supernatants to obtain the extract;

[0058] (5) Concentrate and desalinize the above-mentioned extract by using an ultrafilter. When the volume of the extract is reduced to one-fifth of the original volume, it is a concentrated solution, wherein the molecular weight cut-off of the ...

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Abstract

The invention discloses an active glycoprotein extracted from purslane and a preparation method thereof. The method comprises the following steps: first, extracting active glycoprotein in purslane by using a NaCl solution to obtain an extract; then concentrating and desalting the extract by an ultrafiltration device to obtain a concentrated liquid; then precipitating the concentrated liquid with absolute ethyl alcohol; and finally freeze-drying to obtain the active glycoprotein. The invention has the beneficial effect that the active glycoprotein obtained by the method has high yield and high purity; and compared with the prior art, the active glycoprotein has greatly improved activity in inhibiting oxygen free radicals and scavenging hydroxyl radicals.

Description

Technical field [0001] The invention relates to the extraction of active glycoproteins, in particular to an active glycoprotein extracted from purslane and a preparation method thereof. Background technique [0002] Purslane is a wild plant widely distributed in most areas of my country. Its stems and leaves are edible. Each 100g of fresh purslane contains about 2.4g of protein, 0.5g of fat, 3g of carbohydrates, and 1g of fiber. It also contains effective ingredients such as Vb1, Vb2, VC, VE, carotene, and flavonoids, which have high nutritional value and medicinal value. [0003] Experiments have proved that both Portulaca oleracea polysaccharides and glycoproteins have strong antioxidant and oxygen free radical scavenging capabilities, and can be used to treat enteritis, dysentery, appendicitis, mastitis and other serious diseases. External use can treat erysipelas and snake bites. , Known as "natural antibiotics", at the same time, Portulaca polysaccharides and glycoproteins can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K1/14
CPCC07K14/415
Inventor 孙锋张宽朝阮飞
Owner ANHUI AGRICULTURAL UNIVERSITY
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